Selective medium for primary isolation of members of the tribe Proteeae
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu hodnotící studie, časopisecké články
PubMed
11097023
DOI
10.1007/bf02825652
Knihovny.cz E-zdroje
- MeSH
- bakteriální infekce mikrobiologie MeSH
- druhová specificita MeSH
- infekce spojené se zdravotní péčí mikrobiologie MeSH
- kultivační média * chemie MeSH
- lidé MeSH
- Morganella růst a vývoj izolace a purifikace MeSH
- potravinářská mikrobiologie MeSH
- Proteobacteria klasifikace růst a vývoj izolace a purifikace MeSH
- Proteus růst a vývoj izolace a purifikace MeSH
- Providencia růst a vývoj izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- Názvy látek
- kultivační média * MeSH
A selective Proteeae medium (SPM) for isolation and preliminary detection of species of genera Proteus, Morganella, and Providencia was evaluated. The SPM contains tryptose phosphate agar with phenolphthalein monophosphate (as substrate for phosphatase activity), bile salts and polymyxin B (as inhibitors). The selectivity of the SPM was tested by the ecometric method of quality assurance of culture media. Fourteen reference cultures of enterobacteria and fifty-four strains of Proteeae were tested for their absolute growth index (AGI). Ninety-five percent of tested Proteeae strains display an AGI above 2.5. The detected phosphatase activity proved to be able to discriminate colonies of members of the tribe Proteeae. The ability of SPM for primary isolation of members of Proteeae was tested on food and clinical material and 94 strains were isolated. In addition, the SPM was employed in routine practice of clinical microbiology. From 1016 clinical samples (stool, urine, vaginal and urethral swabs), 57 strains of Proteeae were detected by the SPM in contrast to 35 strains by the routine procedure. The difference amounts to nearly 40%.
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