Was demonstrate the protection ability against reactive oxygen species afforded to S. cerevisiae (wild-type strain SP4 and its mutants deficient in major antioxidant enzymes--catalase T and A, CuZnSOD) by PYA and PYE, new groups of phenolic antioxidants (quaternary ammonium salts of dihydrocinnamic acid amino esters with different alkyl chains; synthesized in this laboratory). The survival of strains exposed to the lipophilic oxidation inducers tert-butyl hydroperoxide (TBHP) and 1,1'-azobis(4-cyclohexane carbonitrile) (ACCN) with or without antioxidant pretreatment was determined by plating. S. cerevisiae mutant deficient in SOD was found to be hypersensitive to TBHP and ACCN while the sensitivity of the strain deficient in catalase T and A was about the same as in the wild-type strain. A 1-h preincubation of cells of both the wild-type and the mutant strains with the phenolic antioxidants prior to exposure to TBHP or ACCN substantially increased the cell survival. The magnitude of protection depended on the strain and the length of the alkyl chain of the antioxidant; the best average protection against TBHP was provided by PYE and PYA compounds with 12- and 16-membered alkyl chains whereas PYE-8 and PYA-12 derivatives afforded the best average protection against ACCN.

Institute of Microbiology University of Wrocław Poland

Zobrazit více v PubMed

Z Naturforsch C J Biosci. 1998 May-Jun;53(5-6):425-30 PubMed

J Biol Chem. 1996 Aug 30;271(35):21167-76 PubMed

Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):7885-90 PubMed

Free Radic Biol Med. 1995 Oct;19(4):523-8 PubMed

J Bacteriol. 1998 Feb;180(3):483-90 PubMed

J Biol Chem. 1996 Jun 7;271(23):13422-9 PubMed

Adv Exp Med Biol. 1994;366:165-9 PubMed

Folia Microbiol (Praha). 1995;40(2):131-52 PubMed

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