Heparin-binding proteins of human seminal plasma homologous with boar spermadhesins
Jazyk angličtina Země Irsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11543852
DOI
10.1016/s0165-0378(01)00072-9
PII: S0165037801000729
Knihovny.cz E-zdroje
- MeSH
- ELISA MeSH
- heparin izolace a purifikace metabolismus MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- peptidové fragmenty chemie metabolismus MeSH
- prasata MeSH
- proteiny semenné plazmy * MeSH
- proteiny izolace a purifikace metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvenční homologie aminokyselin * MeSH
- sperma chemie MeSH
- transportní proteiny * chemie metabolismus MeSH
- vazba proteinů MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- heparin MeSH
- peptidové fragmenty MeSH
- proteiny semenné plazmy * MeSH
- proteiny MeSH
- spermadhesin MeSH Prohlížeč
- transportní proteiny * MeSH
Protein homologues to boar seminal plasma spermadhesins with the N-terminal sequence AQN (AQN spermadhesins) and with the N-terminal sequence AWN (AWN spermadhesins) were detected in human seminal plasma and characterized. They were isolated as heparin-binding (HB) proteins from human seminal plasma by affinity chromatography on heparin-Sepharose and then separated into 12 fractions (HB1-HB12) by RP HPLC or into four major fractions (HB-I-HB-IV) by gel filtration. Rabbit antibody against boar seminal plasma AQN 1 spermadhesin cross-reacted with 10-14 kDa proteins of fraction HB7, and antibody against AWN 1 spermadhesin cross-reacted with 11-14 kDa proteins of fractions HB9 and HB11. Both antibodies interacted with 10-14 kDa proteins in fractions HB-I and HB-II. The N-terminal amino acid sequence (1)AQNKG(5)... was determined in the 14 kDa protein of fraction HB-I cross-reacting with AQN 1 antibodies. A component detected among 10-14 kDa proteins of HB7 cross-reacting with rabbit antiserum against AQN 1 had the N-terminal sequence (1)GELKFVTLVFAVGDYE(16), which is similar to the sequence of a fragment of prostatic acid phosphatase. Lactoferrin and its fragments were immunodetected with rabbit antibody against human milk lactoferrin in fractions HB7-HB11. This was proved by N-terminal sequencing of a lactoferrin fragment immunodetected in fraction HB7. N-terminal amino acid sequence analysis of the dominant component of fraction HB2 revealed the presence of a fragment of semenogelin I.
Citace poskytuje Crossref.org
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