Effects of prostaglandin E2 and nitric oxide inhibitors on the expression of interleukin-10, interleukin-12 and MHC class-II molecules in Mycobacterium microti-infected and interferon-gamma-treated mouse peritoneal macrophages
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11702410
DOI
10.1007/bf02818541
Knihovny.cz E-resources
- MeSH
- Antineoplastic Agents pharmacology MeSH
- Dinoprostone antagonists & inhibitors metabolism MeSH
- Histocompatibility Antigens Class II biosynthesis MeSH
- Indomethacin pharmacology MeSH
- Cyclooxygenase Inhibitors pharmacology MeSH
- Interferon-gamma pharmacology MeSH
- Interleukin-10 biosynthesis MeSH
- Interleukin-12 biosynthesis MeSH
- Mycobacterium Infections immunology metabolism MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- omega-N-Methylarginine pharmacology MeSH
- Nitric Oxide antagonists & inhibitors metabolism MeSH
- Macrophages, Peritoneal drug effects metabolism microbiology MeSH
- Teprotide pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antineoplastic Agents MeSH
- Dinoprostone MeSH
- Histocompatibility Antigens Class II MeSH
- Indomethacin MeSH
- Cyclooxygenase Inhibitors MeSH
- Interferon-gamma MeSH
- Interleukin-10 MeSH
- Interleukin-12 MeSH
- omega-N-Methylarginine MeSH
- Nitric Oxide MeSH
- Teprotide MeSH
Mycobacterium microti-infected mouse peritoneal macrophages produced high amounts of prostaglandin E2 (PGE2) and nitric oxide (NO) when activated with interferon-gamma (IFN-gamma). In order to understand the relation between PGE2 and NO production and the expression of interleukin-12 (IL-12), interleukin-10 (IL-10) and MHC class-II (Ia) molecules by M. microti-infected and IFN-gamma-stimulated macrophages, we analyzed the level of these molecules in the presence or absence of PGE2 and NO inhibitors. Addition of NG-methyl-L-arginine (L-NMA) and indomethacin (IM) caused a significant increase in IL-12 level (2.6- and 1.9-fold, respectively) whereas IL-10 level decreased by 88 and 56%, respectively, relative to M. microti-infected and IFN-gamma-treated control macrophages. Enhanced PGE2 and NO upregulated IL-10 expression and down-regulated IL-12 and MHC class-II (Ia) expression in M. microti-infected and IFN-gamma-treated mouse peritoneal macrophages.
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