Monoclonal antibody to human chronic myeloid leukemia cell line MOLM-7 specifically reacts with an antigen of apoptotic cells
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11734303
DOI
10.1016/s0145-2126(01)00097-2
PII: S0145212601000972
Knihovny.cz E-resources
- MeSH
- Antigens, Neoplasm immunology MeSH
- Antineoplastic Agents, Phytogenic toxicity MeSH
- Apoptosis immunology MeSH
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive genetics immunology MeSH
- DNA, Neoplasm metabolism MeSH
- Fluorescent Antibody Technique, Indirect MeSH
- Camptothecin toxicity MeSH
- Humans MeSH
- Multiple Myeloma immunology MeSH
- Antibodies, Monoclonal immunology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Tumor Cells, Cultured immunology MeSH
- Necrosis MeSH
- Propidium metabolism MeSH
- Flow Cytometry MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Neoplasm MeSH
- Antineoplastic Agents, Phytogenic MeSH
- DNA, Neoplasm MeSH
- Camptothecin MeSH
- Antibodies, Monoclonal MeSH
- Propidium MeSH
Monoclonal antibody 2E12 was prepared by immunization of mice with fresh cells of chronic myeloid leukemia cell line MOLM-7. A panel of 15 leukemic cell lines (myeloid, promyelocytic, erythroid, B and T lymphoid) and numerous cultured patient's leukemia and myeloma cells were tested for reactivity with 2E12 antibody. A subset of cells in all cell lines and various number of patient's cells cultivated for 10 days or more were 2E12 positive. KG-1 and HL-60 cell lines were treated by camptothecin (CAM) (5 microg/ml, 4 h), washed and further cultivated without CAM. After 24 and 48 h in culture a considerable increase of 2E12 positivity was detected both in KG-1 and HL-60 cells, which well correlated with the increase of APO2.7 positivity and the sub-G1 peaks. The 2E12 positive cells were morphologically the same as cells in PCD, possibly apoptosis. We suggest that the 2E12 antibody detects a strong antigen on apoptotic cells which could be a part of the signaling process for ingestion by phagocytes.
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