Microdissection techniques for cancer analysis
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
11871858
Knihovny.cz E-resources
- MeSH
- Cytogenetics instrumentation methods MeSH
- Dissection instrumentation methods MeSH
- DNA, Neoplasm analysis MeSH
- DNA, Complementary genetics MeSH
- Humans MeSH
- Micromanipulation instrumentation methods MeSH
- Neoplastic Stem Cells chemistry MeSH
- Neoplasm Proteins analysis MeSH
- Neoplasms genetics pathology MeSH
- Disease Progression MeSH
- RNA, Neoplasm analysis MeSH
- Oligonucleotide Array Sequence Analysis MeSH
- Cell Separation methods MeSH
- Gene Expression Profiling * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- DNA, Neoplasm MeSH
- DNA, Complementary MeSH
- Neoplasm Proteins MeSH
- RNA, Neoplasm MeSH
One difficulty in studying molecular changes of tumours has been the inability to isolate DNA and RNA from a homogeneous cell population. The combination of several new technologies should help overcome these hurdles. Microdissection is a technique for rapid and easy procurement of a pure cellular subpopulation away from its complex tissue milieu. Laser-assisted microdissection has recently been identified as a quick, simple and effective method by which microdissection of complex tissue specimens can be routinely performed for molecular analysis. With the advent of laser microdissection, cDNA libraries can be developed from pure cells obtained directly from stained neoplastic tissue, and microarrays of thousands of genes can now be used to examine gene expression in microdissected tumour tissue samples. This review will concentrate on the application of different microdissection techniques in the area of cancer research.
