Constant current chronopotentiometric stripping analysis (CPSA) in combination with adsorptive transfer stripping (AdTS) technique was used to study the rabbit liver metallothionein (MT) on a hanging mercury drop electrode (HMDE). Metallothionein yielded a distinct, sharp chronopotentiometric signal at very negative potentials (about -1.7 V), also known as the "peak H". The height and potential of this peak were dependent on experimental conditions, such as buffer composition, pH, and the presence oxygen in solutions. The peak H was highest in borate buffer with pH close to the isoelectric point (pI) of MT. The chronopotentiometric results contribute to a deeper understanding of the nature of catalytic hydrogen evolution and demonstrate the usefulness of the peak H in peptide and protein research.

Department of Theoretical and Physical Chemistry Faculty of Science Masaryk University Kotlárská 2 611 37 Brno Czech Republic

Citace poskytuje Crossref.org

Zeptomole electrochemical detection of metallothioneins

Changes in metallothionein level in rat hepatic tissue after administration of natural mouldy wheat

A Determination of Metallothionein in Larvae of Freshwater Midges (Chironomus riparius) Using Brdicka Reaction

Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues

Influence of Cadmium(II) Ions and Brewery Sludge on Metallothionein Level in Earthworms (Eisenia fetida) - Bio- transforming of Toxic Wastes

Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species