Early cytokine response of gnotobiotic piglets to Salmonella enterica serotype typhimurium
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cytokines biosynthesis blood MeSH
- Enzyme-Linked Immunosorbent Assay veterinary MeSH
- Germ-Free Life immunology MeSH
- Ileum immunology microbiology MeSH
- Interferon-gamma blood MeSH
- Swine, Miniature * MeSH
- Swine MeSH
- Salmonella typhimurium immunology MeSH
- Salmonella Infections, Animal blood immunology MeSH
- Tumor Necrosis Factor-alpha analysis MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cytokines MeSH
- Interferon-gamma MeSH
- Tumor Necrosis Factor-alpha MeSH
Cytokine response against Salmonella Typhimurium is traditionally studied in conventional animals. Germ-free animals, however, enable to study response against infection without background effect of other microorganisms. Plasma and ileal inflammatory cytokines in germ-free piglets orally infected with virulent LT2 strain or, with a non-virulent SF1591 rough mutant were quantified by ELISA. In plasma and ileal washes, IFN-gamma levels significantly increased in both infected groups. TNF-alpha and IL-18 were mostly missing in plasma 24 h after infection. In the ileum, IFN-gamma, TNF-alpha, and IL-1beta were induced mainly by the virulent strain, whereas IL-18 was induced in highest quantity by non-virulent Salmonella. These data confirmed an important role of IFN-gamma, as well as other inflammatory cytokines in early stage of salmonellosis.
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