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Detection of Shiga toxins, intimin and enterohemolysin in Escherichia coli strains isolated from children in eastern Slovakia

Folia microbiologica. 2002 ; 47 (2) : 185-8.

Folia Microbiol (Praha)
ISSN 0015-5632
Source

Language English Country United States Media print

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid12058400

PubMed 12058400
DOI 10.1007/bf02817680
Knihovny.cz E-resources

MeSH
Adhesins, Bacterial analysis MeSH
Child MeSH
Escherichia coli chemistry isolation & purification MeSH
Feces microbiology MeSH
Hemolysin Proteins analysis MeSH
Escherichia coli Infections microbiology MeSH
Humans MeSH
Polymerase Chain Reaction MeSH
Child, Preschool MeSH
Escherichia coli Proteins * MeSH
Shiga Toxins analysis MeSH
Carrier Proteins analysis MeSH
Check Tag
Child MeSH
Humans MeSH
Child, Preschool MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Geographicals
Slovakia MeSH
Names of Substances
Adhesins, Bacterial MeSH
eaeA protein, E coli MeSH Browser
enterohemolysin MeSH Browser
Hemolysin Proteins MeSH
Escherichia coli Proteins * MeSH
Shiga Toxins MeSH
Carrier Proteins MeSH

Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin 1 and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin 1 and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence of E. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated.

Institute of Medical Microbiology Medical Faculty P J Safárik University Kosice Slovakia

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