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PubMed

Záznam pochází z PubMed

The receptor for the subgroup C avian sarcoma and leukosis viruses, Tvc, is related to mammalian butyrophilins, members of the immunoglobulin superfamily

Journal of virology. 2005 Aug ; 79 (16) : 10408-19.

Jazyk angličtina Země Spojené státy americké Médium print

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem, Research Support, U.S. Gov't, P.H.S.

Perzistentní odkaz https://www.medvik.cz/link/pmid16051833

Grantová podpora
R01 AI048682 NIAID NIH HHS - United States
AI48682 NIAID NIH HHS - United States

Online Plný text

PubMed 16051833
PubMed Central PMC1182627
DOI 10.1128/jvi.79.16.10408-10419.2005
PII: 79/16/10408
Knihovny.cz E-zdroje

MeSH
butyrofiliny MeSH
klonování DNA MeSH
kultivované buňky MeSH
kur domácí MeSH
membránové glykoproteiny fyziologie MeSH
molekulární sekvence - údaje MeSH
sekvence aminokyselin MeSH
sekvence nukleotidů MeSH
terminační kodon MeSH
virové receptory chemie genetika fyziologie MeSH
virus ptačí leukózy fyziologie MeSH
viry ptačího sarkomu fyziologie MeSH
zvířata MeSH
Check Tag
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Research Support, N.I.H., Extramural MeSH
Research Support, U.S. Gov't, P.H.S. MeSH
Názvy látek
butyrofiliny MeSH
membránové glykoproteiny MeSH
terminační kodon MeSH
virové receptory MeSH

The five highly related envelope subgroups of the avian sarcoma and leukosis viruses (ASLVs), subgroup A [ASLV(A)] to ASLV(E), are thought to have evolved from an ancestral envelope glycoprotein yet utilize different cellular proteins as receptors. Alleles encoding the subgroup A ASLV receptors (Tva), members of the low-density lipoprotein receptor family, and the subgroup B, D, and E ASLV receptors (Tvb), members of the tumor necrosis factor receptor family, have been identified and cloned. However, alleles encoding the subgroup C ASLV receptors (Tvc) have not been cloned. Previously, we established a genetic linkage between tvc and several other nearby genetic markers on chicken chromosome 28, including tva. In this study, we used this information to clone the tvc gene and identify the Tvc receptor. A bacterial artificial chromosome containing a portion of chicken chromosome 28 that conferred susceptibility to ASLV(C) infection was identified. The tvc gene was identified on this genomic DNA fragment and encodes a 488-amino-acid protein most closely related to mammalian butyrophilins, members of the immunoglobulin protein family. We subsequently cloned cDNAs encoding Tvc that confer susceptibility to infection by subgroup C viruses in chicken cells resistant to ASLV(C) infection and in mammalian cells that do not normally express functional ASLV receptors. In addition, normally susceptible chicken DT40 cells were resistant to ASLV(C) infection after both tvc alleles were disrupted by homologous recombination. Tvc binds the ASLV(C) envelope glycoproteins with low-nanomolar affinity, an affinity similar to that of binding of Tva and Tvb with their respective envelope glycoproteins. We have also identified a mutation in the tvc gene in line L15 chickens that explains why this line is resistant to ASLV(C) infection.

Department of Cellular and Viral Genetics Institute of Molecular Genetics Academy of Sciences of the Czech Republic Prague

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