Human immunodeficiency virus 1 strains resistant to nucleoside inhibitors of reverse transcriptase in isolates from the Czech Republic as monitored by line probe assay and nucleotide sequencing
Jazyk angličtina Země Švýcarsko Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, P.H.S.
Grantová podpora
3D43TW00915
FIC NIH HHS - United States
PubMed
12083326
Knihovny.cz E-zdroje
- MeSH
- dideoxycytidin farmakologie terapeutické užití MeSH
- dideoxyinosin farmakologie terapeutické užití MeSH
- fylogeneze MeSH
- HIV infekce farmakoterapie genetika imunologie MeSH
- HIV reverzní transkriptasa antagonisté a inhibitory MeSH
- HIV-1 účinky léků genetika izolace a purifikace MeSH
- inhibitory reverzní transkriptasy farmakologie terapeutické užití MeSH
- kodon MeSH
- lamivudin farmakologie terapeutické užití MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- molekulární sondy - techniky * MeSH
- mutace MeSH
- prevalence MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- virová léková rezistence genetika MeSH
- zidovudin farmakologie terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- srovnávací studie MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
- Názvy látek
- dideoxycytidin MeSH
- dideoxyinosin MeSH
- HIV reverzní transkriptasa MeSH
- inhibitory reverzní transkriptasy MeSH
- kodon MeSH
- lamivudin MeSH
- zidovudin MeSH
The genetic resistance to nucleoside inhibitors of the reverse transcriptase (RT) of human immunodeficiency virus I (HIV-1) isolates in the Czech Republic was examined by a line probe assay (LiPA) and nucleotide sequencing. The results of LiPA analysis of 294 blood specimens obtained from 156 patients revealed a high incidence of mutations in the RT gene related to resistance to various drugs (67.3%) in various combinations. Mutations in RT gene (M41L, K70R and T215Y/F) conferring the resistance to zidovudine (ZDV) were most frequent (62.6%), that (M184V) responsible for the resistance to lamivudine (3TC) was less frequent (33.7%), while those linked to the resistance to dideoxyinosine (ddl) and dideoxyinosine together with dideoxycytidine (ddl/ddC) were rather rare (6.5% and 5.1%, respectively). LiPA gave a high rate of uninterpretable results due to codon hybridization failure, especially in HIV-1 isolates of non-B subtype. Thirty-two specimens were analyzed also by direct sequencing of a part of RT gene. The results obtained by LiPA and the sequencing were highly concordant for codons successfully analyzed by both methods, but the sequencing provided information also about the codons that could not be analyzed by LiPA. A high prevalence of resistant strains in the Czech Republic and their heterogeneity justifies a regular HIV-1 resistance testing. LiPA turned out as a fast, powerful and most reliable tool for such a purpose. However, due to an increasing diversity of HIV-1 strains circulating in the Czech Republic, LiPA cannot replace the nucleotide sequence analysis.