Human immunodeficiency virus 1 strains resistant to nucleoside inhibitors of reverse transcriptase in isolates from the Czech Republic as monitored by line probe assay and nucleotide sequencing
Language English Country Switzerland Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
Grant support
3D43TW00915
FIC NIH HHS - United States
PubMed
12083326
Knihovny.cz E-resources
- MeSH
- Zalcitabine pharmacology therapeutic use MeSH
- Didanosine pharmacology therapeutic use MeSH
- Phylogeny MeSH
- HIV Infections drug therapy genetics immunology MeSH
- HIV Reverse Transcriptase antagonists & inhibitors MeSH
- HIV-1 drug effects genetics isolation & purification MeSH
- Reverse Transcriptase Inhibitors pharmacology therapeutic use MeSH
- Codon MeSH
- Lamivudine pharmacology therapeutic use MeSH
- Humans MeSH
- Molecular Sequence Data MeSH
- Molecular Probe Techniques * MeSH
- Mutation MeSH
- Prevalence MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Drug Resistance, Viral genetics MeSH
- Zidovudine pharmacology therapeutic use MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Comparative Study MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- Zalcitabine MeSH
- Didanosine MeSH
- HIV Reverse Transcriptase MeSH
- Reverse Transcriptase Inhibitors MeSH
- Codon MeSH
- Lamivudine MeSH
- Zidovudine MeSH
The genetic resistance to nucleoside inhibitors of the reverse transcriptase (RT) of human immunodeficiency virus I (HIV-1) isolates in the Czech Republic was examined by a line probe assay (LiPA) and nucleotide sequencing. The results of LiPA analysis of 294 blood specimens obtained from 156 patients revealed a high incidence of mutations in the RT gene related to resistance to various drugs (67.3%) in various combinations. Mutations in RT gene (M41L, K70R and T215Y/F) conferring the resistance to zidovudine (ZDV) were most frequent (62.6%), that (M184V) responsible for the resistance to lamivudine (3TC) was less frequent (33.7%), while those linked to the resistance to dideoxyinosine (ddl) and dideoxyinosine together with dideoxycytidine (ddl/ddC) were rather rare (6.5% and 5.1%, respectively). LiPA gave a high rate of uninterpretable results due to codon hybridization failure, especially in HIV-1 isolates of non-B subtype. Thirty-two specimens were analyzed also by direct sequencing of a part of RT gene. The results obtained by LiPA and the sequencing were highly concordant for codons successfully analyzed by both methods, but the sequencing provided information also about the codons that could not be analyzed by LiPA. A high prevalence of resistant strains in the Czech Republic and their heterogeneity justifies a regular HIV-1 resistance testing. LiPA turned out as a fast, powerful and most reliable tool for such a purpose. However, due to an increasing diversity of HIV-1 strains circulating in the Czech Republic, LiPA cannot replace the nucleotide sequence analysis.