Decolorization of synthetic textile dyes by lignin peroxidase of Phanerochaete chrysosporium
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12094739
DOI
10.1007/bf02817653
Knihovny.cz E-resources
- MeSH
- Azadirachta MeSH
- Color MeSH
- Coloring Agents metabolism MeSH
- Benzyl Alcohols metabolism MeSH
- Biodegradation, Environmental MeSH
- Bioreactors MeSH
- Hydrogen-Ion Concentration MeSH
- Culture Media MeSH
- Lignin metabolism MeSH
- Peroxidases metabolism MeSH
- Phanerochaete enzymology MeSH
- Substrate Specificity MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Coloring Agents MeSH
- Benzyl Alcohols MeSH
- Culture Media MeSH
- lignin peroxidase MeSH Browser
- Lignin MeSH
- manganese peroxidase MeSH Browser
- Peroxidases MeSH
- veratryl alcohol MeSH Browser
Neem hull waste (containing a high amount of lignin and other phenolic compounds) was used for lignin peroxidase production by Phanerochaete chrysosporum under solid-state fermentation conditions. Maximum decolorization achieved by partially purified lignin peroxidase was 80% for Porocion Brilliant Blue HGR, 83 for Ranocid Fast Blue, 70 for Acid Red 119 and 61 for Navidol Fast Black MSRL. The effects of different concentrations of veratryl alcohol, hydrogen peroxide, enzyme and dye on the efficiency of decolorization have been investigated. Maximum decolorization efficiency was observed at 0.2 and 0.4 mmol/L hydrogen peroxide, 2.5 mmol/L veratryl alcohol and pH 5.0 after a 1-h reaction, using 50 ppm of dyes and 9.96 mkat/L of enzyme.
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