Computer-assisted quantitative analysis of immunofluorescence staining of the extracellular matrix in rat dorsal and ventral spinal roots
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12553705
DOI
10.1078/0065-1281-00664
PII: S0065-1281(04)70141-5
Knihovny.cz E-zdroje
- MeSH
- chondroitinsulfát proteoglykany analýza MeSH
- extracelulární matrix - proteiny * analýza MeSH
- extracelulární matrix chemie MeSH
- fibronektiny analýza MeSH
- fluorescenční protilátková technika nepřímá metody MeSH
- krysa rodu Rattus MeSH
- míšní kořeny chemie cytologie MeSH
- periferní nervy chemie cytologie MeSH
- počítačové zpracování obrazu metody MeSH
- potkani Wistar MeSH
- tenascin analýza MeSH
- thrombospondiny analýza MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chondroitinsulfát proteoglykany MeSH
- extracelulární matrix - proteiny * MeSH
- fibronektiny MeSH
- tenascin MeSH
- thrombospondiny MeSH
The endoneurial extracellular matrix (ECM) is produced by Schwann cells and fibroblasts under the control of axons. Dorsal and ventral spinal roots contain different types of axons, but information is not available on differences in the composition of their ECM. A comparison was made of the intensity of immunofluorescence staining of chondroitin sulfate proteoglycan, fibronectin, tenascin and thrombospondin in the endoneurial ECM of rat dorsal and ventral spinal roots. Sections of dorsal and ventral roots were incubated simultaneously for indirect immunofluorescence detection of the epitopes studied. Brightness of immunofluorescence staining was assessed by computer-assisted image analysis using interactive segmentation of digitized images to select areas to be analyzed. Our results revealed quantitative differences in the composition of endoneurial ECM of spinal dorsal and ventral roots, probably due to the presence of different types of axons. The ECM composition of the endoneurium in dorsal and ventral roots may be related with the creation of extrinsic conditions that support differential regeneration of afferent and motor axons after injury.
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