Fibre type composition of soleus and extensor digitorum longus muscles in normal female inbred Lewis rats
Language English Country Germany Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
12553710
DOI
10.1078/0065-1281-00660
PII: S0065-1281(04)70146-4
Knihovny.cz E-resources
- MeSH
- Adenosine Triphosphatases metabolism MeSH
- Species Specificity MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Immunohistochemistry MeSH
- Muscle, Skeletal cytology metabolism MeSH
- Rats MeSH
- Rats, Inbred Lew MeSH
- Protein Isoforms analysis metabolism MeSH
- Muscle Fibers, Slow-Twitch classification cytology metabolism MeSH
- Muscle Fibers, Fast-Twitch classification cytology metabolism MeSH
- Myosin Heavy Chains analysis metabolism MeSH
- Hindlimb MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Adenosine Triphosphatases MeSH
- Protein Isoforms MeSH
- Myosin Heavy Chains MeSH
We have analysed the fibre type composition of soleus and extensor digitorum longus (EDL) muscles of normal female 4-6-month-old inbred Lewis rats. This rat strain is used in our ongoing study of the effects of thyroid hormone on myosin heavy chain (MyHC) isoform expression. On the basis of the mATPase reaction, soleus muscles contained 96.1 +/- 2.9% of type 1 fibres supplemented by 2A fibres. EDL muscles contained type 1 (5.5 +/- 1.0%), type 2A (18.8 +/- 1.7%) and type 2B (75.7 +/- 2.2%) fibres. Immunohistochemical analysis and SDS gel electrophoresis confirmed that most fibres in the soleus muscle expressed the type 1 (slow) MyHC isoform and that only a small proportion of fibres expressed the fast 2a MyHC isoform. Immunohistochemical analysis and SDS gel electrophoresis demonstrated that almost half of the 2B fibres of EDL muscles expressed the 2x/d MyHC isoform. In both muscle types, many fibres expressed more than one MyHC isoform. The content of slow fibres in the soleus muscle of female inbred Lewis rats was slightly higher than that reported for Wistar rats, but was considerably higher than that of Sprague-Dawley rats, whereas substantial differences were not found in the proportion of slow and fast fibre types in EDL muscles in these strains.
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