Primers ITS1, ITS2 and ITS4 detect the intraspecies variability in the internal transcribed spacers and 5.8S rRNA gene region in clinical isolates of fungi
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
12800508
DOI
10.1007/bf02930961
Knihovny.cz E-zdroje
- MeSH
- Candida klasifikace genetika MeSH
- DNA fungální analýza MeSH
- DNA primery * MeSH
- druhová specificita MeSH
- elektroforéza kapilární MeSH
- genetická variace * MeSH
- geny rRNA MeSH
- houby klasifikace genetika MeSH
- lidé MeSH
- mezerníky ribozomální DNA analýza genetika MeSH
- mykologické určovací techniky MeSH
- mykózy mikrobiologie MeSH
- polymerázová řetězová reakce metody MeSH
- RNA ribozomální 5.8S analýza genetika MeSH
- Saccharomyces cerevisiae klasifikace genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA fungální MeSH
- DNA primery * MeSH
- mezerníky ribozomální DNA MeSH
- RNA ribozomální 5.8S MeSH
Restriction fragment length polymorphism analysis of the 5.8S rRNA gene and the internal transcribed spacers (ITS1 and ITS2) was used for examination of 66 isolates belonging to 19 species. Intraspecies variability was found in the examined region of 11 species (Candida albicans, C. catenulata, C. colliculosa, C. glabrata, C. kefyr, C. melinii, C. parapsilosis, C. guillermondii, C. solanii, C. tropicalis, Saccharomyces cerevisiae). Region of ITS-5.8S rDNA was amplified using the primers ITS1 and ITS4. The amplicons were digested by HaeIII, HinfI and CfoI. The recognized intraspecies variability was confirmed in the second step, in which the shorter fragments of this region were amplified using primers ITS1 and ITS2 and analyzed by capillary electrophoresis. Considerable intraspecific variability renders this method unsuitable for species identification, whereas it can be useful for epidemiological tracing of isolates.
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