Characterization of Cryptococcus neoformans var. neoformans serotype A and A/D in samples from Egypt
Language English Country United States Media print
Document type Evaluation Study, Journal Article
PubMed
12800514
DOI
10.1007/bf02930967
Knihovny.cz E-resources
- MeSH
- Cryptococcus neoformans classification genetics isolation & purification MeSH
- Child MeSH
- DNA, Fungal blood cerebrospinal fluid MeSH
- Adult MeSH
- Meningitis, Cryptococcal diagnosis microbiology MeSH
- Cryptococcosis diagnosis microbiology MeSH
- Latex Fixation Tests MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Mycological Typing Techniques MeSH
- Intestinal Diseases diagnosis microbiology MeSH
- Polymerase Chain Reaction methods MeSH
- Polysaccharides blood cerebrospinal fluid MeSH
- Child, Preschool MeSH
- Sensitivity and Specificity MeSH
- Serotyping MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Child, Preschool MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Geographicals
- Egypt MeSH
- Names of Substances
- cryptococcal polysaccharide MeSH Browser
- DNA, Fungal MeSH
- Polysaccharides MeSH
The cryptococcal polysaccharide antigen was detected in 10 cerebrospinal fluid (CSF) and 23 serum samples from cryptococcal meningitis and intestinal cryptococcosis by the cryptococcal antigen latex agglutination system (CALAS). CALAS titers in CSF and serum samples of cryptococcal meningitis ranged over 8-2048 and 32-2048, respectively, while in cases of intestinal cryptococcosis, serum titers ranged over 8-2048. The isolates of yeast Cryptococcus neoformans were determined to be of serotype A or of the A/D pair. The total leukocyte count and biochemical parameters in CSF were significantly increased as indicators of microbial infection. Furthermore, the in vitro change of the teleomorph (sexual state) to the anamorph (asexual state) was also detected and the teleomorph state changed in vivo to the encapsulated anamoph state which is more virulent during infection in vivo than the yeast-like noncapsulated form. Two primers for internal transcribed spacer (ITS) regions of ribosomal DNA were used for molecular detection of C. neoformans. After PCR amplification, a DNA band of 415 bp, visualized on agarose gel, indicated the presence of C. neoformans cells in the tested CSF and serum samples. The primer sensitivity was also characterized using purified yeast chromosomal DNA as template; it was about 20 pg or more chromosomal DNA which represents about 10 cells of C. neoformans. The primers were also specific for ITS regions of C. neoformans and gave negative results with Candida albicans and E. coli chromosomal DNA templates.
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