Xstir polymorphism and absence of sex linkage in Xenopus laevis ME2 gene
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12859020
Knihovny.cz E-resources
- MeSH
- Exons MeSH
- Genetic Linkage MeSH
- Genetic Markers MeSH
- Introns MeSH
- DNA, Complementary metabolism MeSH
- Malate Dehydrogenase genetics MeSH
- Molecular Sequence Data MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Polymorphism, Genetic * MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Homology, Amino Acid MeSH
- Sex Factors MeSH
- Xenopus laevis genetics MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- D-malate dehydrogenase (decarboxylating) MeSH Browser
- Genetic Markers MeSH
- DNA, Complementary MeSH
- Malate Dehydrogenase MeSH
A fragment of ME2 cDNA from exon 2 to exon 11 was sequenced and the sequence submitted to GenBank. Analysis of the intron, probably intron 13, revealed a polymorphism which is due to the presence of tandem repetitions of Xstir elements. Genetic analysis of the parents and the offspring showed a standard distribution of intron variants. This distribution was not dependent on sex. We conclude, contrary to previous reports, that the ME2 gene is not linked to sex. Consequently, the Xstir polymorphism can be used as a tool for genetic analysis.
