Cyclin-dependent kinase inhibitor, roscovitine, in combination with exogenous cytokinin, N6-benzyladenine, causes increase of cis-cytokinins in immobilized tobacco cells
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Adenine analogs & derivatives pharmacology MeSH
- Alginates MeSH
- Benzyl Compounds MeSH
- Cyclin-Dependent Kinases antagonists & inhibitors MeSH
- Cytokinins biosynthesis MeSH
- Cells, Immobilized drug effects metabolism MeSH
- Isopentenyladenosine biosynthesis MeSH
- Kinetin MeSH
- Cells, Cultured MeSH
- Glucuronic Acid MeSH
- Hexuronic Acids MeSH
- Purines pharmacology MeSH
- Reproducibility of Results MeSH
- Roscovitine MeSH
- Sensitivity and Specificity MeSH
- Nicotiana drug effects metabolism MeSH
- Cell Survival MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenine MeSH
- Alginates MeSH
- benzylaminopurine MeSH Browser
- Benzyl Compounds MeSH
- Cyclin-Dependent Kinases MeSH
- Cytokinins MeSH
- Isopentenyladenosine MeSH
- Kinetin MeSH
- Glucuronic Acid MeSH
- Hexuronic Acids MeSH
- Purines MeSH
- Roscovitine MeSH
Dynamics of the response of tobacco cells (line BY-2) to exogenous cytokinin, N6-benzyladenine, and cyclin-dependent kinase inhibitor, roscovitine, was followed using alginate-immobilized cells packed into a column. N6-Benzyladenine (1.25 microM) increased the synthesis of the physiologically-active endogenous cytokinin, isopentenyladenosine, in the effluent up to 0.1 nM. Simultaneously, conversion of the excess of endogenous cytokinins to biologically inactive derivatives of cis-zeatin occurred, up to 0.8 nM. Roscovitine (50 microM) further increased cis-cytokinins, up to 2.2 nM.
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