In this work we established differential pulse anodic stripping voltammetry (DPASV) as the tool for analysis of lead in the plant cell culture. For the cultivation procedure, lead in Pb(II)-ethylenediaminetetraacetic acid (Pb-EDTA) chelate has been used. The detection limit of lead was found at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract (20 pg Pb(II)/mg cells). For determination of cysteine-rich peptides, voltammetry in differential mode (DPV) in cobalt(III)-containing ammonia buffer (Brdicka reaction) was used. In this short communication, we present suitable voltammetric techniques for the physiological study of lead and thiols in plant cell culture.

Department of Botany and Plant Physiology Mendel University of Agriculture and Forestry Zemedelska 1 613 00 Brno Czech Republic

Citace poskytuje Crossref.org

Electrochemical microsensors for the detection of cadmium(II) and lead(II) ions in plants

Ferric reducing antioxidant power and square wave voltammetry for assay of low molecular weight antioxidants in blood plasma: performance and comparison of methods

Sunflower Plants as Bioindicators of Environmental Pollution with Lead (II) Ions

Electrochemical Determination of Low Molecular Mass Thiols Content in Potatoes (Solanum tuberosum) Cultivated in the Presence of Various Sulphur Forms and Infected by Late Blight (Phytophora infestans)

Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species