Electrochemical determination of lead and glutathione in a plant cell culture
Language English Country Netherlands Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15110301
DOI
10.1016/j.bioelechem.2003.08.007
PII: S1567539404000635
Knihovny.cz E-resources
- MeSH
- Cell Culture Techniques methods MeSH
- Electrochemistry methods MeSH
- Glutathione analysis metabolism MeSH
- Hydrogen-Ion Concentration MeSH
- Cells, Cultured MeSH
- Lead analysis metabolism MeSH
- Picea metabolism MeSH
- Sulfhydryl Compounds analysis metabolism MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Glutathione MeSH
- Lead MeSH
- Sulfhydryl Compounds MeSH
In this work we established differential pulse anodic stripping voltammetry (DPASV) as the tool for analysis of lead in the plant cell culture. For the cultivation procedure, lead in Pb(II)-ethylenediaminetetraacetic acid (Pb-EDTA) chelate has been used. The detection limit of lead was found at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract (20 pg Pb(II)/mg cells). For determination of cysteine-rich peptides, voltammetry in differential mode (DPV) in cobalt(III)-containing ammonia buffer (Brdicka reaction) was used. In this short communication, we present suitable voltammetric techniques for the physiological study of lead and thiols in plant cell culture.
References provided by Crossref.org
Electrochemical microsensors for the detection of cadmium(II) and lead(II) ions in plants
Sunflower Plants as Bioindicators of Environmental Pollution with Lead (II) Ions
Shapes of Differential Pulse Voltammograms and Level of Metallothionein at Different Animal Species
