Determination of minimal regrowth concentration (MRC) in clinical isolates of various biofilm-forming bacteria
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15114870
DOI
10.1007/bf02931650
Knihovny.cz E-resources
- MeSH
- Acinetobacter baumannii drug effects growth & development MeSH
- Amikacin pharmacology MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Bacteria drug effects growth & development MeSH
- Bacterial Adhesion drug effects MeSH
- Drug Resistance, Bacterial MeSH
- Biofilms drug effects growth & development MeSH
- Chloramphenicol pharmacology MeSH
- Ciprofloxacin pharmacology MeSH
- Escherichia coli drug effects growth & development MeSH
- Klebsiella pneumoniae drug effects growth & development MeSH
- Clindamycin pharmacology MeSH
- Lactams pharmacology MeSH
- Microbial Sensitivity Tests methods MeSH
- Netilmicin pharmacology MeSH
- Predictive Value of Tests MeSH
- Pseudomonas aeruginosa drug effects growth & development MeSH
- Staphylococcus drug effects growth & development MeSH
- Teicoplanin pharmacology MeSH
- Tetracycline pharmacology MeSH
- Vancomycin pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amikacin MeSH
- Anti-Bacterial Agents MeSH
- Chloramphenicol MeSH
- Ciprofloxacin MeSH
- Clindamycin MeSH
- Lactams MeSH
- Netilmicin MeSH
- Teicoplanin MeSH
- Tetracycline MeSH
- Vancomycin MeSH
Based on the ability to attach to polymeric surfaces, the formation of biofilms was determined in 5 wild-type strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumanii, Escherichia coli, Staphylococcus warneri). Using modified Christensen method, minimum regrowth concentration (MRC) of piperacillin, piperacillin-tazobactam, cefoperazon, ceftazidim, cefepim, meronem, ciprofloxacin, netilmicin and amikacin for Gram-negative and of ampicillin-sulbactam, chloramphenicol, tetracycline, clindamycin, vancomycin and teicoplanin for Gram-positive bacteria was estimated in trypticase-soy broth medium after a 1-d growth on polystyrene microtiter plates. Adherent bacterial populations exhibited reduced antimicrobial susceptibility, which was not shown in submerged cultures. Our results indicate that MRC can predict therapeutic outcome of antibiotic treatment better than the minimum inhibitory concentration tests commonly used.
See more in PubMed
J Bacteriol. 2000 May;182(10):2675-9 PubMed
J Ind Microbiol Biotechnol. 1999 Jul;23(1):713-7 PubMed
Science. 1999 May 21;284(5418):1318-22 PubMed
J Bone Joint Surg Br. 1998 Jul;80(4):568-72 PubMed
J Infect Dis. 1994 May;169(5):1042-9 PubMed
J Clin Microbiol. 1985 Dec;22(6):996-1006 PubMed
Lancet. 2003 Jan 11;361(9352):130-5 PubMed
Infect Immun. 1999 Oct;67(10):5427-33 PubMed
Infect Immun. 1982 Jul;37(1):318-26 PubMed
J Antimicrob Chemother. 1999 Jul;44(1):43-55 PubMed
Int J Antimicrob Agents. 2003 Jan;21(1):8-12 PubMed
Lancet. 2001 Jul 14;358(9276):135-8 PubMed
J Clin Microbiol. 1999 Jun;37(6):1771-6 PubMed
Eur J Clin Microbiol Infect Dis. 2001 Jan;20(1):20-6 PubMed
J Antimicrob Chemother. 2001 Dec;48(6):793-801 PubMed
Infect Immun. 1998 Oct;66(10):4711-20 PubMed
Prog Drug Res. 1991;37:91-105 PubMed
Med Microbiol Immunol. 2002 Oct;191(2):107-14 PubMed
J Clin Microbiol. 2001 Feb;39(2):750-3 PubMed
Antibiotic synergy against biofilm-forming Pseudomonas aeruginosa
Clinical pseudomonas aeruginosa: potential factors of pathogenicity and resistance to antimicrobials
Effect of pH on production of virulence factors by biofilm cells of Pseudomonas aeruginosa
