Adjuvant effect of dendritic cells transduced with recombinant vaccinia virus expressing HPV16-E7 is inhibited by co-expression of IL12
Language English Country Greece Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15138603
Knihovny.cz E-resources
- MeSH
- Adjuvants, Immunologic MeSH
- Dendritic Cells immunology MeSH
- Neoplasms, Experimental immunology therapy virology MeSH
- Genetic Therapy * MeSH
- Genetic Vectors MeSH
- Immunization MeSH
- Immunotherapy * MeSH
- Papillomavirus Infections genetics immunology therapy MeSH
- Interleukin-12 genetics immunology therapeutic use MeSH
- Humans MeSH
- Survival Rate MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Oncogene Proteins, Viral genetics immunology therapeutic use MeSH
- Papillomaviridae physiology MeSH
- Papillomavirus E7 Proteins MeSH
- Antigen Presentation MeSH
- Cancer Vaccines immunology therapeutic use MeSH
- Th1 Cells immunology MeSH
- Transduction, Genetic MeSH
- Vaccinia virus genetics MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Adjuvants, Immunologic MeSH
- Interleukin-12 MeSH
- oncogene protein E7, Human papillomavirus type 16 MeSH Browser
- Oncogene Proteins, Viral MeSH
- Papillomavirus E7 Proteins MeSH
- Cancer Vaccines MeSH
Dendritic cells (DC) enhanced the immunogenicity of recombinant vaccinia viruses (rVV) expressing the E7 protein of HPV16, a tumor-associated antigen (TAA). Immunization with DC transduced by rVV generated from strain Praha or MVA induced better protection against the growth of transplanted TC-1 tumors in C57Bl/6 mice than did immunization with either of these rVVs administered alone by the same route. Interestingly, DC transduced with a double recombinant vaccinia virus expressing E7 protein together with the Th1-polarizing cytokine IL12, which has been shown to enhance the cellular response in several other systems, induced lower anti-tumor immunity than DC transduced with rVV expressing E7 protein alone. The inhibitory effect mediated by IL12 on immunization with rVV-infected DC was dose-dependent and was observed after immunization with DC transduced with IL12-expressing rVV even at low multiplicity.
