Identification of bacteriophage types and their carriage in Staphylococcus aureus
Jazyk angličtina Země Rakousko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- DNA primery MeSH
- DNA virů analýza chemie izolace a purifikace MeSH
- genom virový MeSH
- lyzogenie MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce MeSH
- profágy klasifikace genetika izolace a purifikace MeSH
- sekvenční analýza DNA MeSH
- stafylokokové bakteriofágy klasifikace genetika izolace a purifikace MeSH
- Staphylococcus aureus virologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA primery MeSH
- DNA virů MeSH
Conserved genomic sequences distinctive of Staphylococcus aureus phage types 3A, 11, 77, 187 and Twort, representative of phage serogroups A, B, F, L and D, were identified and characterized. PCR primers designed for the above sequences were used for development of a multiplex PCR assay which enabled us not only to classify all phages of the International Typing Set plus 16 additional phages, but also to detect prophages in S. aureus genomes. One to four different prophages were unambiguously detected in experimentally lysogenized S. aureus strains, and substantial variation in prophage content was found in 176 S. aureus clinical strains of different provenance. In addition, by using a comparative genomics approach, all the prophages in the S. aureus genomes sequenced to date could be revealed and classified.
Citace poskytuje Crossref.org
GENBANK
AF513855, AF513856, AF513857, AF515457, AY158000, AY362039
