Isolation of genomic DNA using magnetic cobalt ferrite and silica particles
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15595531
PII: S0021-9673(04)01448-7
Knihovny.cz E-resources
- MeSH
- Adsorption MeSH
- DNA chemistry isolation & purification MeSH
- Electrophoresis, Agar Gel MeSH
- Cobalt chemistry MeSH
- Chickens MeSH
- Magnetics MeSH
- Microscopy, Electron, Scanning MeSH
- Silicon Dioxide chemistry MeSH
- Polymerase Chain Reaction MeSH
- Ferric Compounds chemistry MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA MeSH
- ferrite MeSH Browser
- Cobalt MeSH
- Silicon Dioxide MeSH
- Ferric Compounds MeSH
Adsorption separation techniques as an alternative to laborious traditional methods (e.g., based on phenol extraction procedure) have been applied for DNA purification. In this work we used two types of particles: silica and cobalt ferrite (unmodified or modified with a reagent containing weakly basic aminoethyl groups, aminophenyl groups, or alginic acid). DNA from chicken erythrocytes and DNA isolated from bacteria Lactococcus lactis were used for testing of adsorption/desorption properties of particles. The cobalt ferrite particles modified with different reagents were used for isolation of PCR-ready bacterial DNA from different dairy products.
