Neoparamoeba branchiphila n. sp., and related species of the genus Neoparamoeba Page, 1987: morphological and molecular characterization of selected strains
Language English Country Great Britain, England Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15660793
DOI
10.1111/j.1365-2761.2004.00600.x
PII: JFD600
Knihovny.cz E-resources
- MeSH
- Principal Component Analysis MeSH
- Analysis of Variance MeSH
- DNA Primers MeSH
- Species Specificity MeSH
- Phylogeny * MeSH
- Lobosea genetics ultrastructure MeSH
- Models, Genetic MeSH
- Molecular Sequence Data MeSH
- Likelihood Functions MeSH
- RNA, Ribosomal genetics MeSH
- Salmo salar microbiology MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Microscopy, Electron, Transmission MeSH
- Gills microbiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- DNA Primers MeSH
- RNA, Ribosomal MeSH
A total of 18 Neoparamoeba strains were characterized both morphologically and using the SSU rRNA gene sequences as molecular markers. Nine were isolated from gills of farmed Atlantic salmon, Salmo salar L., six from sediments sampled in areas of sea-cage farms and three from net material of sea-cages. The newly obtained sequences extended substantially the dataset of Neoparamoeba strains available for phylogenetic analyses, which were used to infer taxonomic relatedness among 32 strains morphologically assigned to this genus. In addition to the N. pemaquidensis and N. aestuarina clades, phylogenetic analyses clearly distinguished a third clade with sequences from six strains. Members of this clade are characterized as representatives of a new species, N. branchiphila n. sp. The diagnostic primers for the identification of this species are introduced.
References provided by Crossref.org
