Nutrient stress causes akinete differentiation in cyanobacterium Anabaena torulosa with concomitant increase in nitrogen reserve substances
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15702545
DOI
10.1007/bf02931533
Knihovny.cz E-resources
- MeSH
- Anabaena growth & development metabolism MeSH
- Bacterial Proteins metabolism MeSH
- Nitrogen metabolism MeSH
- Glycogen metabolism MeSH
- Culture Media MeSH
- Plant Proteins metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- cyanophycin MeSH Browser
- Nitrogen MeSH
- Glycogen MeSH
- Culture Media MeSH
- Plant Proteins MeSH
Addition of nitrogen source (nitrate), carbon sources (acetate, citrate and fructose), depletion of nutrients (phosphate-free nitrate medium), dilution of medium (2, 4 and 8 times diluted nitrate medium) under unaerated conditions induced akinete differentiation in Anabaena torulosa. Aerated cultures under the same conditions did not differentiate akinetes. The amounts of reserve metabolites--glycogen and cyanophycin (multi-L-arginyl-poly-L-aspartic acid) granule polypeptide (CGP)--were determined in unaerated and aerated cultures, and at different stages of growth and akinete differentiation. The addition of nitrate, acetate, citrate and fructose under unaerated conditions resulted in the accumulation of glycogen and CGP in higher amounts after 4 d (akinete initiation); the CGP content further changed at mature free akinetes phase. Higher accumulation of reserve products was also observed under nutrient deficiency (phosphate-depleted or diluted media) after 4 d of cultivation. Under aerated conditions reserve product accumulation was considerably lower. Thus a low accumulation of reserve products in aerated cultures showed that aeration probably somehow relieves the organism from a nutritional stress.
See more in PubMed
Annu Rev Microbiol. 1968;22:15-46 PubMed
Folia Microbiol (Praha). 2000;45(5):434-8 PubMed
Folia Microbiol (Praha). 2004;49(1):64-70 PubMed
Folia Microbiol (Praha). 2002;47(5):527-34 PubMed
Folia Microbiol (Praha). 2003;48(4):501-9 PubMed
J Bacteriol. 1980 Feb;141(2):687-93 PubMed
Annu Rev Microbiol. 1984;38:1-25 PubMed
Z Allg Mikrobiol. 1980;20(7):459-63 PubMed
Plant Physiol. 1955 Jul;30(4):366-72 PubMed
Arch Mikrobiol. 1972;86(1):25-38 PubMed
Folia Microbiol (Praha). 2002;47(1):61-7 PubMed
Z Allg Mikrobiol. 1979;19(8):571-5 PubMed
Biochim Biophys Acta. 1976 Feb 13;422(2):407-18 PubMed
J Bacteriol. 1977 Feb;129(2):1154-5 PubMed
Plant Physiol. 1981 Apr;67(4):716-9 PubMed
Arch Mikrobiol. 1969;67(1):62-70 PubMed
Z Allg Mikrobiol. 1980;20(10):653-6 PubMed
Metabolites produced by cyanobacteria belonging to several species of the family Nostocaceae
Motility in Oscillatoria salina as affected by different factors
Survival of blue-green and green algae under stress conditions
