PKB/AKT is involved in resumption of meiosis in mouse oocytes
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
Grant support
HD22681
NICHD NIH HHS - United States
PubMed
15842198
DOI
10.1042/bc20050020
PII: BC20050020
Knihovny.cz E-resources
- MeSH
- Enzyme Activation MeSH
- Centrosome metabolism MeSH
- Chromones pharmacology MeSH
- Phosphorylation MeSH
- 4-Butyrolactone analogs & derivatives pharmacology MeSH
- Nuclear Envelope metabolism MeSH
- Okadaic Acid pharmacology MeSH
- Meiosis * MeSH
- Morpholines pharmacology MeSH
- Mice MeSH
- Oocytes drug effects physiology MeSH
- CDC2 Protein Kinase metabolism MeSH
- Proto-Oncogene Proteins c-akt antagonists & inhibitors physiology MeSH
- Serine metabolism MeSH
- In Vitro Techniques MeSH
- Threonine metabolism MeSH
- Protein Transport MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
- Names of Substances
- 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one MeSH Browser
- butyrolactone I MeSH Browser
- Chromones MeSH
- 4-Butyrolactone MeSH
- Okadaic Acid MeSH
- Morpholines MeSH
- CDC2 Protein Kinase MeSH
- Proto-Oncogene Proteins c-akt MeSH
- Serine MeSH
- Threonine MeSH
BACKGROUND INFORMATION: In fully grown mouse oocytes, a decrease in cAMP concentration precedes and is linked to CDK1 (cyclin-dependent kinase 1) activation. The molecular mechanism for this coupling, however, is not defined. PKB (protein kinase B, also called AKT) is implicated in CDK1 activation in lower species. During resumption of meiosis in starfish oocytes, MYT1, a negative regulator of CDK1, is phosphorylated by PKB in an inhibitory manner. It can imply that PKB is also involved in CDK1 activation in mammalian oocytes. RESULTS: We monitored activation of PKB and CDK1 during maturation of mouse oocytes. PKB phosphorylation and activation preceded GVBD (germinal vesicle breakdown) in oocytes maturing either in vitro or in vivo. Activation was transient and PKB activity was markedly reduced when virtually all of the oocytes had undergone GVBD. PKB activation was independent of CDK1 activity, because although butyrolactone I prevented CDK1 activation and GVBD, PKB was nevertheless transiently phosphorylated and activated. LY-294002, an inhibitor of phosphoinositide 3-kinase-PKB signalling, suppressed activation of PKB and CDK1 as well as resumption of meiosis. OA (okadaic acid)-sensitive phosphatases are involved in PKB-activity regulation, because OA induced PKB hyperphosphorylation. During resumption of meiosis, PKB phosphorylated on Ser(473) is associated with nuclear membrane and centrosome, whereas PKB phosphorylated on Thr(308) is localized on centrosome only. CONCLUSIONS: The results of the present paper indicate that PKB is involved in CDK1 activation and resumption of meiosis in mouse oocytes. The presence of phosphorylated PKB on centrosome at the time of GVBD suggests its important role for an initial CDK1 activation.
References provided by Crossref.org
A Role of PI3K/Akt Signaling in Oocyte Maturation and Early Embryo Development
Multiple Roles of PLK1 in Mitosis and Meiosis
SGK1 is essential for meiotic resumption in mammalian oocytes
Aurora kinase A controls meiosis I progression in mouse oocytes
CDC25A phosphatase controls meiosis I progression in mouse oocytes
