PKB/AKT is involved in resumption of meiosis in mouse oocytes
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
Grantová podpora
HD22681
NICHD NIH HHS - United States
PubMed
15842198
DOI
10.1042/bc20050020
PII: BC20050020
Knihovny.cz E-zdroje
- MeSH
- aktivace enzymů MeSH
- centrozom metabolismus MeSH
- chromony farmakologie MeSH
- fosforylace MeSH
- gama-butyrolakton analogy a deriváty farmakologie MeSH
- jaderný obal metabolismus MeSH
- kyselina okadaová farmakologie MeSH
- meióza * MeSH
- morfoliny farmakologie MeSH
- myši MeSH
- oocyty účinky léků fyziologie MeSH
- proteinkinasa CDC2 metabolismus MeSH
- protoonkogenní proteiny c-akt antagonisté a inhibitory fyziologie MeSH
- serin metabolismus MeSH
- techniky in vitro MeSH
- threonin metabolismus MeSH
- transport proteinů MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Názvy látek
- 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one MeSH Prohlížeč
- butyrolactone I MeSH Prohlížeč
- chromony MeSH
- gama-butyrolakton MeSH
- kyselina okadaová MeSH
- morfoliny MeSH
- proteinkinasa CDC2 MeSH
- protoonkogenní proteiny c-akt MeSH
- serin MeSH
- threonin MeSH
BACKGROUND INFORMATION: In fully grown mouse oocytes, a decrease in cAMP concentration precedes and is linked to CDK1 (cyclin-dependent kinase 1) activation. The molecular mechanism for this coupling, however, is not defined. PKB (protein kinase B, also called AKT) is implicated in CDK1 activation in lower species. During resumption of meiosis in starfish oocytes, MYT1, a negative regulator of CDK1, is phosphorylated by PKB in an inhibitory manner. It can imply that PKB is also involved in CDK1 activation in mammalian oocytes. RESULTS: We monitored activation of PKB and CDK1 during maturation of mouse oocytes. PKB phosphorylation and activation preceded GVBD (germinal vesicle breakdown) in oocytes maturing either in vitro or in vivo. Activation was transient and PKB activity was markedly reduced when virtually all of the oocytes had undergone GVBD. PKB activation was independent of CDK1 activity, because although butyrolactone I prevented CDK1 activation and GVBD, PKB was nevertheless transiently phosphorylated and activated. LY-294002, an inhibitor of phosphoinositide 3-kinase-PKB signalling, suppressed activation of PKB and CDK1 as well as resumption of meiosis. OA (okadaic acid)-sensitive phosphatases are involved in PKB-activity regulation, because OA induced PKB hyperphosphorylation. During resumption of meiosis, PKB phosphorylated on Ser(473) is associated with nuclear membrane and centrosome, whereas PKB phosphorylated on Thr(308) is localized on centrosome only. CONCLUSIONS: The results of the present paper indicate that PKB is involved in CDK1 activation and resumption of meiosis in mouse oocytes. The presence of phosphorylated PKB on centrosome at the time of GVBD suggests its important role for an initial CDK1 activation.
Citace poskytuje Crossref.org
A Role of PI3K/Akt Signaling in Oocyte Maturation and Early Embryo Development
Multiple Roles of PLK1 in Mitosis and Meiosis
SGK1 is essential for meiotic resumption in mammalian oocytes
Aurora kinase A controls meiosis I progression in mouse oocytes
CDC25A phosphatase controls meiosis I progression in mouse oocytes
