Comparison of different kinds of probes used for analysis of variant telomeric sequences
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
15963624
DOI
10.1016/j.bpc.2005.05.008
PII: S0301-4622(05)00124-9
Knihovny.cz E-zdroje
- MeSH
- DNA sondy analýza genetika MeSH
- genetická variace genetika MeSH
- genom genetika MeSH
- klonování DNA MeSH
- lidé MeSH
- sekvence nukleotidů MeSH
- telomery genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- DNA sondy MeSH
In this work we aimed to compare and critically evaluate results obtained by different types of probes used for hybridisation to detect variant telomeric sequences with respect to their reliability and information value. Using slot-blot hybridisation we investigated three types of probes (oligonucleotides, cloned fragments and concatenated probes) under various conditions of hybridisation and washing. The concatenated probes exhibited the highest specificity although all three types are suitable for hybridisation of telomeric sequences under appropriate experimental conditions. We demonstrate how understanding generated from these data enables interpretation of hybridisation patterns of oligonucleotide probes to genomic DNAs.
Citace poskytuje Crossref.org
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