Standard pathways involved in the regulation of telomere stability do not contribute to gradual telomere elongation observed in the course of A. thaliana calli propagation. Genetic and epigenetic changes accompanying the culturing of plant cells have frequently been reported. Here we aimed to characterize the telomere homeostasis during long term callus propagation. While in Arabidopsis thaliana calli gradual telomere elongation was observed, telomeres were stable in Nicotiana tabacum and N. sylvestris cultures. Telomere elongation during callus propagation is thus not a general feature of plant cells. The long telomere phenotype in Arabidopsis calli was correlated neither with changes in telomerase activity nor with activation of alternative mechanisms of telomere elongation. The dynamics of telomere length changes was maintained in mutant calli with loss of function of important epigenetic modifiers but compromised in the presence of epigenetically active drug zebularine. To examine whether the cell culture-induced disruption of telomere homeostasis is associated with the modulated structure of chromosome ends, epigenetic properties of telomere chromatin were analysed. Albeit distinct changes in epigenetic modifications of telomere histones were observed, these were broadly stochastic. Our results show that contrary to animal cells, the structure and function of plant telomeres is not determined significantly by the epigenetic character of telomere chromatin. Set of differentially transcribed genes was identified in calli, but considering the known telomere- or telomerase-related functions of respective proteins, none of these changes per se was apparently related to the elongated telomere phenotype. Based on our data, we propose that the disruption in telomere homeostasis in Arabidopsis calli arises from the interplay of multiple factors, as a part of reprogramming of plant cells to long-term culture conditions.

Institute of Biophysics Academy of Sciences of the Czech Republic v v i 612 65 Brno Czech Republic

Institute of Organic Chemistry and Biochemistry Academy of Sciences of the Czech Republic v v i Prague Czech Republic

Laboratory of Functional Genomics and Proteomics Faculty of Science National Centre for Biomolecular Research Masaryk University 625 00 Brno Czech Republic

Mendel Centre for Plant Genomics and Proteomics Central European Institute of Technology Masaryk University 625 00 Brno Czech Republic

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Oncogene. 2002 Jan 21;21(4):598-610 PubMed

Symp Soc Exp Biol. 1957;11:118-30 PubMed

Nat Genet. 1999 May;22(1):94-7 PubMed

Nat Biotechnol. 2013 Aug;31(8):686-8 PubMed

Mol Cell. 2009 Oct 23;36(2):207-18 PubMed

Plant J. 2003 May;34(3):283-91 PubMed

Genes Dev. 2012 Nov 15;26(22):2512-23 PubMed

Mol Cell. 2007 Jul 6;27(1):163-9 PubMed

Nucleic Acids Res. 2014 Mar;42(5):2919-31 PubMed

Biol Cell. 2009 Jul;101(7):375-92, 1 p following 392 PubMed

Curr Opin Genet Dev. 2010 Apr;20(2):190-6 PubMed

Dev Biol. 2007 Jun 15;306(2):838-46 PubMed

Plant Cell. 1998 Oct;10(10):1691-8 PubMed

Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1711-6 PubMed

EMBO J. 2002 Jun 3;21(11):2819-26 PubMed

Genes Chromosomes Cancer. 2011 Oct;50(10):823-9 PubMed

Biophys Chem. 2005 Oct 3;117(3):225-31 PubMed

Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14422-7 PubMed

Chromosoma. 2010 Oct;119(5):485-93 PubMed

Proc Natl Acad Sci U S A. 2011 Jan 4;108(1):73-78 PubMed

FEBS Lett. 1996 Aug 12;391(3):307-9 PubMed

Methods. 2017 Feb 1;114:74-84 PubMed

Development. 2009 Feb;136(4):509-23 PubMed

Plant Cell. 2015 Jun;27(6):1788-800 PubMed

Front Plant Sci. 2014 Nov 04;5:593 PubMed

Jpn J Genet. 1994 Aug;69(4):361-70 PubMed

Blood Cells Mol Dis. 2000 Oct;26(5):534-9 PubMed

J Vis Exp. 2014 Dec 19;(94):null PubMed

Nucleic Acids Res. 1991 Sep 11;19(17):4780 PubMed

Plant Mol Biol. 2011 Nov;77(4-5):371-80 PubMed

Dev Cell. 2010 Mar 16;18(3):463-71 PubMed

Mol Gen Genet. 1995 Jun 10;247(5):633-8 PubMed

Nat Rev Genet. 2008 Jun;9(6):465-76 PubMed

PLoS Genet. 2010 Jun 10;6(6):e1000986 PubMed

Cell. 1988 Apr 8;53(1):127-36 PubMed

Nat Biotechnol. 2009 Dec;27(12):1181-5 PubMed

Mol Cells. 2016 Jun 30;39(6):484-94 PubMed

Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5222-6 PubMed

Plant Cell. 2007 Jan;19(1):23-31 PubMed

Front Plant Sci. 2016 Jun 28;7:851 PubMed

Nucleic Acids Res. 2012 Nov;40(21):e163 PubMed

Cells. 2017 Jun 19;6(2):null PubMed

EMBO J. 2009 Aug 19;28(16):2323-36 PubMed

J Cell Sci. 2007 Oct 15;120(Pt 20):3678-87 PubMed

Methods Mol Biol. 2005;286:103-10 PubMed

Plant Cell. 2004 Nov;16(11):2910-22 PubMed

Plant Physiol. 2017 Nov;175(3):1158-1174 PubMed

Plant J. 2014 Mar;77(5):770-81 PubMed

Plant J. 2005 Sep;43(5):662-74 PubMed

Nucleic Acids Res. 2011 Mar;39(6):2007-17 PubMed

J Biol Chem. 2001 Jun 22;276(25):22772-8 PubMed

Mol Gen Genet. 1998 Dec;260(5):470-4 PubMed

Plant Cell Rep. 2018 Mar;37(3):501-513 PubMed

Nat Protoc. 2006;1(3):1583-90 PubMed

Plant Mol Biol. 2008 Apr;66(6):637-46 PubMed

Crit Rev Biochem Mol Biol. 2017 Feb;52(1):57-73 PubMed

FEBS Lett. 2000 Feb 4;467(1):47-51 PubMed

Plant Cell. 2004 Aug;16(8):1959-67 PubMed

Plant J. 2009 Feb;57(3):542-54 PubMed

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