Comparison of different kinds of probes used for analysis of variant telomeric sequences
Language English Country Netherlands Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15963624
DOI
10.1016/j.bpc.2005.05.008
PII: S0301-4622(05)00124-9
Knihovny.cz E-resources
- MeSH
- DNA Probes analysis genetics MeSH
- Genetic Variation genetics MeSH
- Genome genetics MeSH
- Cloning, Molecular MeSH
- Humans MeSH
- Base Sequence MeSH
- Telomere genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- DNA Probes MeSH
In this work we aimed to compare and critically evaluate results obtained by different types of probes used for hybridisation to detect variant telomeric sequences with respect to their reliability and information value. Using slot-blot hybridisation we investigated three types of probes (oligonucleotides, cloned fragments and concatenated probes) under various conditions of hybridisation and washing. The concatenated probes exhibited the highest specificity although all three types are suitable for hybridisation of telomeric sequences under appropriate experimental conditions. We demonstrate how understanding generated from these data enables interpretation of hybridisation patterns of oligonucleotide probes to genomic DNAs.
References provided by Crossref.org
A broad phylogenetic survey unveils the diversity and evolution of telomeres in eukaryotes
Dynamic evolution of telomeric sequences in the green algal order Chlamydomonadales
