Molecular cloning and comparative analysis of fibrinogen-related proteins from the soft tick Ornithodoros moubata and the hard tick Ixodes ricinus
Language English Country Great Britain, England Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15979000
DOI
10.1016/j.ibmb.2005.04.001
PII: S0965-1748(05)00101-3
Knihovny.cz E-resources
- MeSH
- Species Specificity MeSH
- Fibrinogen analogs & derivatives chemistry MeSH
- Phylogeny MeSH
- Ixodes chemistry MeSH
- Cloning, Molecular MeSH
- Molecular Sequence Data MeSH
- Ornithodoros chemistry MeSH
- Amino Acid Sequence MeSH
- Sequence Homology, Amino Acid MeSH
- Sequence Alignment MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Fibrinogen MeSH
Among disease-vectors, the evolution of the tick innate immune system is still lagging when compared to insects. Such an investigation, which was initiated, by first cloning and sequencing lectins associated in the innate immunity of invertebrates and having fibrinogen related domains, helped in the sequencing of cDNA encoding for OMFREP from the soft tick, Ornithodoros moubata. Also obtained were Ixoderin A and Ixoderin B cDNA sequences from the hard tick Ixodes ricinus. Tissue-specific expression of OMFREP showed that it was present primarily in the hemocytes and salivary glands. Ixoderin A besides sharing a similar expression profile was also expressed in the midgut. Both showed significantly high homology to the lectin Dorin M, from O. moubata. Further, phylogenetic comparisons between these molecules of the soft and hard ticks showed their relatedness to Tachylectins 5A and 5B, involved in the innate immunity of Tachypleus tridentatus and ficolins from both vertebrates and invertebrates. Ixoderin B showing tissue-specific expression only in the salivary glands and the sequence displaying certain motif differences in homology point towards a possible function different from the other two molecules. This is the first report of lectin-like sequences, with a fibrinogen-domain, from the hard tick I. ricinus and a preliminary phylogenetic study of these tick sequences with related fibrinogen-domain containing sequences highlights a possible role for them in the innate immunity of the ticks.
References provided by Crossref.org
Tick Immune System: What Is Known, the Interconnections, the Gaps, and the Challenges
A bite so sweet: the glycobiology interface of tick-host-pathogen interactions
Multiple Acquisitions of Pathogen-Derived Francisella Endosymbionts in Soft Ticks
Deep Sequencing Analysis of the Ixodes ricinus Haemocytome
Interaction of the tick immune system with transmitted pathogens
Insight into the sialome of the castor bean tick, Ixodes ricinus