Dimethyl sulfoxide potentiates death receptor-mediated apoptosis in the human myeloid leukemia U937 cell line through enhancement of mitochondrial membrane depolarization
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15998540
DOI
10.1016/j.leukres.2005.05.016
PII: S0145-2126(05)00208-0
Knihovny.cz E-resources
- MeSH
- Adaptor Proteins, Signal Transducing metabolism MeSH
- fas Receptor metabolism MeSH
- Apoptosis drug effects MeSH
- Dimethyl Sulfoxide pharmacology MeSH
- CASP8 and FADD-Like Apoptosis Regulating Protein MeSH
- Intracellular Signaling Peptides and Proteins MeSH
- Caspase 3 MeSH
- Caspase 8 MeSH
- Caspases metabolism MeSH
- Cryoprotective Agents pharmacology MeSH
- Humans MeSH
- Membrane Glycoproteins metabolism MeSH
- Mitochondrial Membranes metabolism pathology MeSH
- Mitochondria metabolism pathology MeSH
- Leukemia, Myeloid metabolism pathology MeSH
- Fas-Associated Death Domain Protein MeSH
- TNF-Related Apoptosis-Inducing Ligand MeSH
- Apoptosis Regulatory Proteins metabolism MeSH
- Proto-Oncogene Proteins c-bcl-2 metabolism MeSH
- Tumor Necrosis Factor-alpha metabolism MeSH
- U937 Cells MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adaptor Proteins, Signal Transducing MeSH
- fas Receptor MeSH
- CASP3 protein, human MeSH Browser
- CASP8 protein, human MeSH Browser
- CFLAR protein, human MeSH Browser
- Dimethyl Sulfoxide MeSH
- FADD protein, human MeSH Browser
- CASP8 and FADD-Like Apoptosis Regulating Protein MeSH
- Intracellular Signaling Peptides and Proteins MeSH
- Caspase 3 MeSH
- Caspase 8 MeSH
- Caspases MeSH
- Cryoprotective Agents MeSH
- Membrane Glycoproteins MeSH
- Fas-Associated Death Domain Protein MeSH
- TNF-Related Apoptosis-Inducing Ligand MeSH
- Apoptosis Regulatory Proteins MeSH
- Proto-Oncogene Proteins c-bcl-2 MeSH
- Tumor Necrosis Factor-alpha MeSH
- TNFSF10 protein, human MeSH Browser
Dimethyl sulfoxide (DMSO) is a widely used prototypical chemical inducer of cell differentiation. In the present study, the effects of DMSO on susceptibility of human myeloid leukemia U937 cells towards ligation of distinct death receptors (DRs) were investigated. DMSO sensitized cells towards induction of apoptosis by anti-Fas antibody, tumour necrosis factor-alpha or Apo2 ligand/TNF-related apoptosis-inducing ligand (TRAIL). Apart from increasing Fas levels, DMSO did not affect expression of proteins in death signal transduction, such as Bcl-2 family proteins, FADD, caspase-3 and -8, the inhibitor of apoptosis proteins (IAPs) or cFLIP(L). However, DMSO significantly potentiated mitochondrial membrane depolarization, suggesting that this mechanism might be involved in sensitisation of myeloid cells to DR-mediated apoptosis.
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