Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16585638
DOI
10.1099/jmm.0.46246-0
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Drug Resistance, Bacterial MeSH
- Bacterial Proteins analysis genetics MeSH
- Child MeSH
- DNA, Bacterial chemistry genetics MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Molecular Sequence Data MeSH
- Novobiocin pharmacology MeSH
- Polymerase Chain Reaction MeSH
- Proteome analysis MeSH
- Ribotyping MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Cluster Analysis MeSH
- Staphylococcal Infections microbiology MeSH
- Staphylococcus classification genetics isolation & purification physiology MeSH
- Superoxide Dismutase genetics MeSH
- Bacterial Typing Techniques MeSH
- Check Tag
- Child MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Bacterial Proteins MeSH
- DNA, Bacterial MeSH
- Novobiocin MeSH
- Proteome MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
- SodA protein, Bacteria MeSH Browser
- Superoxide Dismutase MeSH
A polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. Phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as Staphylococcus xylosus and three strains as ambiguous S. xylosus/Staphylococcus equorum. In contrast to biotyping, ribotyping with EcoRI and HindIII restriction endonucleases and whole-cell protein fingerprinting assigned six analysed strains as S. equorum, and five strains as Staphylococcus succinus. Confirmation of the identification was done by partial 16S rRNA gene sequencing and S. equorum isolates were verified by a PCR assay targeting the sodA gene. From the data it has been implied that ribotyping and whole-cell protein analysis can be used to differentiate between the biochemically almost indistinguishable species S. xylosus, S. equorum and S. succinus. The present study confirms what is believed to be the first occurrence of S. equorum in a relevant human clinical material in the Czech Republic and describes what is believed to be the first-ever isolation of S. succinus from human clinical material.
References provided by Crossref.org
Ribotyping and biotyping of Staphylococcus epidermidis isolated from hospital environment
GENBANK
DQ006829, DQ006830, DQ006831, DQ006832, DQ006833, DQ006834, DQ006835, DQ006836, DQ006837, DQ006838, DQ006839
