Comparison of "high throughput" micromethods for determination of cytochrome P450 activities with classical methods using HPLC for product identification
Language English Country Czech Republic Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
16601787
DOI
10.5507/bp.2005.057
Knihovny.cz E-resources
- MeSH
- Aryl Hydrocarbon Hydroxylases analysis MeSH
- Cytochrome P-450 CYP3A MeSH
- Cytochrome P-450 CYP2A6 MeSH
- Cytochrome P-450 CYP2C9 MeSH
- Diclofenac chemistry MeSH
- Coumarins chemistry MeSH
- Humans MeSH
- Mixed Function Oxygenases analysis MeSH
- Cytochrome P-450 Enzyme System analysis MeSH
- Testosterone chemistry MeSH
- Chromatography, High Pressure Liquid MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Aryl Hydrocarbon Hydroxylases MeSH
- coumarin MeSH Browser
- CYP2A6 protein, human MeSH Browser
- CYP2C9 protein, human MeSH Browser
- CYP3A4 protein, human MeSH Browser
- Cytochrome P-450 CYP3A MeSH
- Cytochrome P-450 CYP2A6 MeSH
- Cytochrome P-450 CYP2C9 MeSH
- Diclofenac MeSH
- Coumarins MeSH
- Mixed Function Oxygenases MeSH
- Cytochrome P-450 Enzyme System MeSH
- Testosterone MeSH
Enzyme activities of the CYP enzymes (CYP3A4, CYP2C9 and CYP2A6) were determined using classical substrates (testosterone, diclofenac and coumarin, respectively) as well as with luminogenic or fluorogenic substrates in micromethod arrangement. The luciferin-based luminogenic substrates for CYP3A4 and CYP2C9 as well as coumarin in micromethod for assay of CYP2A6 activity gave results well comparable with the classical methods with determination of reaction products by the HPLC.
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