Change of the protein p53 electrochemical signal according to its structural form - quick and sensitive distinguishing of native, denatured, and aggregated form of the "guardian of the genome"
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- denaturace proteinů MeSH
- elektrochemie MeSH
- konformace proteinů MeSH
- lidé MeSH
- nádorový supresorový protein p53 chemie MeSH
- průtoková injekční analýza metody MeSH
- senzitivita a specificita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- nádorový supresorový protein p53 MeSH
Presence of mutated and/or structurally modified (e.g., denatured, aggregated) protein p53 form is associated with several disorders such as Alzheimer's disease, Parkinson's disease, prion diseases, and many types of tumours. The aim of this work was to distinguish native, denatured and aggregated form of full-length p53 by flow injection analysis coupled with electrochemical detector (FIA-ED). Firstly FIA-ED method used for protein native form determination was optimized (detection limit 45.8 amol per 5 mul injection; 3 x S/N). In addition the technique was applied to identify p53 structural forms (denatured and aggregated). It was found out that denatured form provides about three times higher electrochemical response (protein structure unfolding, approach of more electroactive centers - aminoacid residues - towards electrode surface) in comparison with native form. On the other hand, aggregated form offers lower response (steric eclipse of electroactive protein parts) when compared with the signal of native form. The obtained data show that we are not only able to sensitively determine native, denatured, and aggregated structural forms of p53 protein but also to distinguish them.
Zobrazit více v PubMed
Semin Cancer Biol. 1994 Jun;5(3):203-10 PubMed
Biochem Biophys Res Commun. 2000 Jan 27;267(3):934-9 PubMed
Nucleic Acids Res. 2002 Nov 15;30(22):4966-74 PubMed
J Natl Cancer Inst. 1993 Jun 16;85(12):965-70 PubMed
Carcinogenesis. 1996 Jun;17(6):1187-98 PubMed
EMBO J. 1998 Jun 15;17(12):3342-50 PubMed
J Cell Sci. 1992 Jan;101 ( Pt 1):183-9 PubMed
Cell Mol Life Sci. 1999 Jan;55(1):28-37 PubMed
Science. 1996 Dec 6;274(5293):1672-7 PubMed
J Biol Chem. 1999 Sep 3;274(36):25749-55 PubMed
J Immunol Methods. 2002 Sep 15;267(2):227-35 PubMed
Histopathology. 2004 Aug;45(2):180-6 PubMed
Diagn Mol Pathol. 1995 Dec;4(4):249-55 PubMed
Science. 1994 Nov 25;266(5189):1376-80 PubMed
Urology. 2004 Apr;63(4):651-5 PubMed
Clin Cancer Res. 2004 Sep 15;10(18 Pt 1):6215-21 PubMed
Anal Bioanal Chem. 2005 Mar;381(6):1167-78 PubMed
Biochim Biophys Acta. 1985 Nov 12;823(1):67-78 PubMed
Nucleic Acids Res. 1998 Jan 1;26(1):205-13 PubMed
Br J Cancer. 1994 Jan;69(1):26-31 PubMed
Biochemistry. 2003 Aug 5;42(30):9022-7 PubMed
Anal Chem. 2004 Oct 1;76(19):5930-6 PubMed
Eur J Cancer. 1998 May;34(6):845-50 PubMed
J Chromatogr A. 1998 May 8;806(1):169-85 PubMed
Bioessays. 1995 Nov;17(11):923-30 PubMed
J Urol. 1997 Mar;157(3):1049-53 PubMed
Proc Natl Acad Sci U S A. 1999 Aug 31;96(18):9989-90 PubMed
Oncogene. 1999 Dec 13;18(53):7621-36 PubMed
Anal Chem. 2003 Jun 1;75(11):2663-9 PubMed
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14338-42 PubMed
Bioelectrochemistry. 2002 Jan;55(1-2):115-8 PubMed
Dis Esophagus. 1999;12(2):128-31 PubMed
Biochem Biophys Res Commun. 2001 Oct 19;288(1):111-5 PubMed
Nat Genet. 1992 Apr;1(1):45-9 PubMed
Anal Chem. 2001 Oct 15;73(20):4801-7 PubMed
Amperometric Sensor for Detection of Chloride Ions
An Electrochemical Detection of Metallothioneins at the Zeptomole Level in Nanolitre Volumes
