Intrahippocampal injection of endothelin-1 in immature rats results in neuronal death, development of epilepsy and behavioral abnormalities later in life
Jazyk angličtina Země Francie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
16836644
DOI
10.1111/j.1460-9568.2006.04910.x
PII: EJN4910
Knihovny.cz E-zdroje
- MeSH
- arteriae cerebrales účinky léků patofyziologie MeSH
- cévní mozková příhoda chemicky indukované komplikace patofyziologie MeSH
- degenerace nervu chemicky indukované patologie patofyziologie MeSH
- elektroencefalografie účinky léků MeSH
- endotelin-1 škodlivé účinky MeSH
- epilepsie chemicky indukované patofyziologie MeSH
- hipokampus účinky léků patologie patofyziologie MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- mozková hypoxie a ischemie chemicky indukované komplikace patofyziologie MeSH
- mozkový krevní oběh účinky léků MeSH
- novorozená zvířata MeSH
- novorozenec MeSH
- poruchy paměti chemicky indukované patofyziologie MeSH
- potkani Wistar MeSH
- rozvrh dávkování léků MeSH
- vazokonstriktory škodlivé účinky MeSH
- věkové faktory MeSH
- vývojové poruchy u dětí chemicky indukované patofyziologie MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- endotelin-1 MeSH
- vazokonstriktory MeSH
The direct injection of endothelin-1 (ET-1) into brain parenchyma was recently suggested as a suitable model of stroke. The present study was designed to assess whether intrahippocampal injection of ET-1 in immature rats causes neurodegeneration and immediate seizures, and results in impairment of motor development, cognitive decline, epilepsy and chronic hippocampal lesion. ET-1 was injected unilaterally into the dorsal hippocampus in doses of 20 or 40 pmol at the age of 12 (P12) or 25 (P25) days. Video-electroencephalographic monitoring performed during 100 min after the injection of ET-1 demonstrated the development of convulsive epileptic seizures in 75-100% of animals of individual age-and-dose groups. Long-term behavioral follow-up did not reveal impairment of motor development in any dose-and-age group. At 2 months after ET-1 injection, impairment of spatial memory occurred only in rats with 40 pmol of ET-1 at P12. At 3 months after ET-1 injection spontaneous electrographic seizures occurred in 62.5-100% animals of both ages with no relation to the dose used. Seizures were always non-convulsive. The total seizure duration per 24 h was higher in the P12 than the P25 group, suggesting more severe epilepsy. The extent of the hippocampal lesion increased with the dose of ET-1 and was significantly higher in the P12 than the P25 group. The severity of the ET-1-induced lesion correlated positively with total seizure duration per 24 h at both ages. Our results document that early intrahippocampal injection of ET-1 results in lesion development and both immediate seizures and chronic epilepsy in either age group. Cognitive impairment occurred only in rats with ET-1 injection at P12.
Citace poskytuje Crossref.org
Epilepsy Research in the Institute of Physiology of the Czech Academy of Sciences in Prague
