High extracellular K(+) evokes changes in voltage-dependent K(+) and Na (+) currents and volume regulation in astrocytes
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- astrocyty fyziologie MeSH
- draslík farmakokinetika MeSH
- draslíkové kanály řízené napětím fyziologie MeSH
- gliový fibrilární kyselý protein metabolismus MeSH
- glióza patofyziologie MeSH
- hypotonické roztoky farmakologie MeSH
- imunohistochemie MeSH
- koncentrace vodíkových iontů MeSH
- krysa rodu Rattus MeSH
- membránové potenciály účinky léků fyziologie MeSH
- metoda terčíkového zámku MeSH
- mícha cytologie MeSH
- osmotický tlak MeSH
- potkani Wistar MeSH
- sodík metabolismus MeSH
- sodíkové kanály fyziologie MeSH
- velikost buňky MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- draslík MeSH
- draslíkové kanály řízené napětím MeSH
- gliový fibrilární kyselý protein MeSH
- hypotonické roztoky MeSH
- sodík MeSH
- sodíkové kanály MeSH
[K(+)](e) increase accompanies many pathological states in the CNS and evokes changes in astrocyte morphology and glial fibrillary acidic protein expression, leading to astrogliosis. Changes in the electrophysiological properties and volume regulation of astrocytes during the early stages of astrocytic activation were studied using the patch-clamp technique in spinal cords from 10-day-old rats after incubation in 50 mM K(+). In complex astrocytes, incubation in high K(+) caused depolarization, an input resistance increase, a decrease in membrane capacitance, and an increase in the current densities (CDs) of voltage-dependent K(+) and Na(+) currents. In passive astrocytes, the reversal potential shifted to more positive values and CDs decreased. No changes were observed in astrocyte precursors. Under hypotonic stress, astrocytes in spinal cords pre-exposed to high K(+) revealed a decreased K(+) accumulation around the cell membrane after a depolarizing prepulse, suggesting altered volume regulation. 3D confocal morphometry and the direct visualization of astrocytes in enhanced green fluorescent protein/glial fibrillary acidic protein mice showed a smaller degree of cell swelling in spinal cords pre-exposed to high K(+) compared to controls. We conclude that exposure to high K(+), an early event leading to astrogliosis, caused not only morphological changes in astrocytes but also changes in their membrane properties and cell volume regulation.
Zobrazit více v PubMed
Pflugers Arch. 1981 Aug;391(2):85-100 PubMed
J Neurophysiol. 1966 Jul;29(4):788-806 PubMed
Glia. 1989;2(1):45-50 PubMed
Neurochem Res. 1999 Feb;24(2):213-8 PubMed
Brain Pathol. 1994 Jul;4(3):239-43 PubMed
Glia. 1995 Oct;15(2):173-87 PubMed
J Neurosci. 1997 Oct 1;17(19):7316-29 PubMed
Annu Rev Physiol. 1979;41:159-77 PubMed
Eur J Neurosci. 1995 Jan 1;7(1):129-42 PubMed
Brain Res. 2000 Apr 17;862(1-2):187-93 PubMed
Eur J Neurosci. 1995 Jun 1;7(6):1188-98 PubMed
Neuroreport. 2000 Sep 28;11(14):3151-5 PubMed
Prog Biophys Mol Biol. 1983;42(2-3):135-89 PubMed
Neuroreport. 1994 Jan 31;5(5):639-41 PubMed
J Neurophysiol. 2001 Apr;85(4):1719-31 PubMed
J Neurosci. 1996 Apr 15;16(8):2532-45 PubMed
J Neurosci. 1999 Sep 15;19(18):8152-62 PubMed
Glia. 1999 Nov;28(2):166-74 PubMed
Glia. 2001 Sep;35(3):189-203 PubMed
Glia. 2001 Jan;33(1):72-86 PubMed
Eur J Neurosci. 2000 Jun;12(6):2087-96 PubMed
Neuroscience. 2000;100(2):431-8 PubMed
Brain Res Brain Res Rev. 2000 Apr;32(2-3):380-412 PubMed
Brain Res. 1995 Mar 20;674(2):314-28 PubMed
J Neurosci Res. 1999 Jun 1;56(5):493-505 PubMed
J Neurochem. 1995 Apr;64(4):1576-84 PubMed
Prog Neurobiol. 2003 Jul;70(4):363-86 PubMed
Glia. 2000 Mar;30(1):27-38 PubMed
Neuroscience. 2004;129(4):1045-56 PubMed
J Cereb Blood Flow Metab. 1995 May;15(3):409-16 PubMed
Brain Res. 1992 Oct 23;594(1):19-30 PubMed
J Neurosci Res. 2001 Jul 15;65(2):129-38 PubMed
J Neurophysiol. 1998 Apr;79(4):2222-6 PubMed
Glia. 1999 Sep;27(3):213-25 PubMed
J Cereb Blood Flow Metab. 1997 Feb;17 (2):191-203 PubMed
Neuroscience. 1997 Dec;81(3):847-60 PubMed
J Neural Transm (Vienna). 2005 Jan;112(1):137-47 PubMed
Glia. 2005 Jun;50(4):427-34 PubMed
Physiol Rev. 2001 Jul;81(3):1065-96 PubMed
Brain Res. 2000 Jul 28;872(1-2):194-8 PubMed
J Neurosci. 2003 Mar 1;23(5):1750-8 PubMed
Glia. 1999 Jan;25(1):56-70 PubMed
J Neurosci Res. 2004 Dec 1;78(5):668-82 PubMed
Brain Res. 2000 May 12;864(2):220-9 PubMed
Glia. 2004 Dec;48(4):311-26 PubMed
Glia. 1994 Jun;11(2):156-72 PubMed
Neurosci Biobehav Rev. 1997 Mar;21(2):135-42 PubMed
Transient astrocyte-like NG2 glia subpopulation emerges solely following permanent brain ischemia
