HPLC quantification of seven quaternary benzo[c]phenanthridine alkaloids in six species of the family Papaveraceae
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17367981
DOI
10.1016/j.jpba.2007.02.005
PII: S0731-7085(07)00124-0
Knihovny.cz E-resources
- MeSH
- Acetonitriles chemistry MeSH
- Alkaloids analysis chemistry MeSH
- Species Specificity MeSH
- Ethanolamines chemistry MeSH
- Phenanthridines analysis chemistry isolation & purification MeSH
- Calibration MeSH
- Hydrogen-Ion Concentration MeSH
- Plant Roots chemistry MeSH
- Plants, Medicinal anatomy & histology chemistry MeSH
- Methanol chemistry MeSH
- Molecular Structure MeSH
- Papaveraceae anatomy & histology chemistry MeSH
- Regression Analysis MeSH
- Reproducibility of Results MeSH
- Plant Extracts chemistry MeSH
- Spectrophotometry, Ultraviolet MeSH
- Water chemistry MeSH
- Chromatography, High Pressure Liquid instrumentation methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- acetonitrile MeSH Browser
- Acetonitriles MeSH
- Alkaloids MeSH
- Ethanolamines MeSH
- Phenanthridines MeSH
- Methanol MeSH
- Plant Extracts MeSH
- triethanolamine MeSH Browser
- Water MeSH
The content of the seven quaternary benzo[c]phenanthridine alkaloids (QBA) sanguinarine (SA), chelerythrine (CHE), chelirubine (CHR), chelilutine (CHL), sanguilutine (SL), sanguirubine (SR) and macarpine (MA) was determined in the underground part of six plant species of the family Papaveraceae (Sanguinaria canadensis L., Dicranostigma lactucoides HOOK.f.et THOMS, Chelidonium majus L., Macleaya cordata (Willd.), Macleaya microcarpa (Maxim) and Stylophorum lasiocarpum (Oliv.)). HPLC method with reversed phase column Synergi Max-RP C-12 Phenomenex was used, mobile phase consisted of heptanesulfonic acid (0.01 mol/l) with triethanolamine (0.1 mol/l) in redistilled water, pH 2.5, acetonitrile gradient 25-60% during 25 min. Detection was performed at 280 nm. The highest content of SA and CHE was found in the roots of D. lactucoides (1.99%, resp. 3.43% of the dried roots). In rhizomes of S. canadensis was their content more then two times lower.
References provided by Crossref.org
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