In vitro genotoxicity of PAH mixtures and organic extract from urban air particles part I: acellular assay
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17442348
DOI
10.1016/j.mrfmmm.2007.03.001
PII: S0027-5107(07)00112-1
Knihovny.cz E-resources
- MeSH
- DNA Adducts analysis MeSH
- Benzo(a)pyrene analysis MeSH
- Carcinogens, Environmental toxicity MeSH
- Rats MeSH
- Air Pollutants toxicity MeSH
- Humans MeSH
- Organic Chemicals toxicity MeSH
- Particulate Matter toxicity MeSH
- Polycyclic Aromatic Hydrocarbons metabolism toxicity MeSH
- Mutagenicity Tests methods MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA Adducts MeSH
- benzo(a)pyrene-DNA adduct MeSH Browser
- Benzo(a)pyrene MeSH
- Carcinogens, Environmental MeSH
- Air Pollutants MeSH
- Organic Chemicals MeSH
- Particulate Matter MeSH
- Polycyclic Aromatic Hydrocarbons MeSH
Acellular assay of calf thymus DNA+/-rat liver microsomal S9 fraction coupled with (32)P-postlabelling was used to study the genotoxic potential of organic compounds bound onto PM10 particles collected in three European cities-Prague (CZ), Kosice (SK) and Sofia (BG) during summer and winter periods. B[a]P alone induced DNA adduct levels ranging from 4.8 to 768 adducts/10(8) nucleotides in the concentration dependent manner. However, a mixture of 8 c-PAHs with equimolar doses of B[a]P induced 3.7-757 adducts/10(8) nucleotides, thus suggesting the inhibition of DNA adduct forming activity by interaction among various PAHs. Comparison of DNA adduct levels induced by various EOMs indicates higher variability among seasons than among localities. DNA adduct levels for Prague collection site varied from 19 to 166 adducts/10(8) nucleotides, for Kosice from 22 to 85 and for Sofia from 6 to 144 adducts/10(8) nucleotides. Bioactivation with S9 microsomal fraction caused 2- to 7-fold increase in DNA adduct levels compared to -S9 samples, suggesting a crucial role of indirectly acting genotoxic EOM components, such as PAHs. We have demonstrated for the first time a significant positive correlation between B[a]P content in EOMs and total DNA adduct levels detected in the EOM treated samples (R=0.83; p=0.04). These results suggest that B[a]P content in EOM is an important factor for the total genotoxic potential of EOM and/or B[a]P is a good indicator of the presence of other genotoxic compounds causing DNA adducts. Even stronger correlation between the content of genotoxic compounds in EOMs and total DNA adduct levels detected (R=0.94; p=0.005) was found when eight c-PAHs were taken into the consideration. Our findings support a hypothesis that a relatively limited number of EOM components is responsible for a major part of its genotoxicity detectable as DNA adducts by (32)P-postlabelling.
References provided by Crossref.org
Evaluation of Fine and Ultrafine Particles Proportion in Airborne Dust in an Industrial Area
Airborne Benzo[a]Pyrene may contribute to divergent Pheno-Endotypes in children
Biomarkers of exposure and effect-interpretation in human risk assessment
