Clinical relevance of the expression of mRNA of MMP-7, MMP-9, TIMP-1, TIMP-2 and CEA tissue samples from colorectal liver metastases
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17992052
DOI
10.1159/000110897
PII: 000110897
Knihovny.cz E-resources
- MeSH
- Adenocarcinoma genetics metabolism mortality secondary surgery MeSH
- Survival Analysis MeSH
- Adult MeSH
- Carcinoembryonic Antigen biosynthesis genetics MeSH
- Colorectal Neoplasms mortality pathology surgery MeSH
- Middle Aged MeSH
- Humans MeSH
- Matrix Metalloproteinase 7 biosynthesis genetics MeSH
- Matrix Metalloproteinase 9 biosynthesis genetics MeSH
- RNA, Messenger biosynthesis MeSH
- Neoplasm Proteins biosynthesis genetics MeSH
- Liver Neoplasms genetics metabolism mortality secondary MeSH
- Disease-Free Survival MeSH
- Prognosis MeSH
- RNA, Neoplasm biosynthesis MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Tissue Inhibitor of Metalloproteinase-1 biosynthesis genetics MeSH
- Tissue Inhibitor of Metalloproteinase-2 biosynthesis genetics MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Carcinoembryonic Antigen MeSH
- Matrix Metalloproteinase 7 MeSH
- Matrix Metalloproteinase 9 MeSH
- RNA, Messenger MeSH
- MMP7 protein, human MeSH Browser
- Neoplasm Proteins MeSH
- RNA, Neoplasm MeSH
- Tissue Inhibitor of Metalloproteinase-1 MeSH
- Tissue Inhibitor of Metalloproteinase-2 MeSH
BACKGROUND: Nowadays we know that survival rates do not differ between repeated and single liver resections for colorectal liver metastases (CLM). To be able to determine patients prone to early recurrence, the use of different markers with a better prognostic value than the routinely employed tumor markers is required. AIM OF STUDY: The aim of our study was to assess mRNA expression of MMP-7, MMP-9, TIMP-1, TIMP-2 and CEA in tissue samples from CLM and their relationship to disease-free interval (DFI) and overall survival (OS). PATIENTS AND METHODS: The liver tumor biopsies were obtained from 40 patients suffering from CLM treated with radical surgery. mRNA expression levels of CEA, MMPs and TIMPs and a housekeeping gene (GAPDH) were quantified using RT-PCR. RESULTS: The increased expression of CEA, MMP-9 and TIMP-1 in CLM was associated with a short DFI and a high tendency to early CLM recurrence. Statistical analysis confirmed CEA, MMP-9 and TIMP-1 expression as prognostic factors of survival. CONCLUSION: This study demonstrated the importance of CEA, MMP-9 and TIMP-1 in the prognostication of DFI and OS.
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