Applications of a microplate reader in yeast physiology research
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18072596
DOI
10.2144/000112620
PII: 000112620
Knihovny.cz E-resources
- MeSH
- Bromcresol Purple metabolism MeSH
- Bromcresol Green metabolism MeSH
- Photometry instrumentation methods MeSH
- Calibration MeSH
- Hydrogen-Ion Concentration MeSH
- Culture Media MeSH
- Acids metabolism MeSH
- Saccharomyces cerevisiae cytology growth & development physiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bromcresol Purple MeSH
- Bromcresol Green MeSH
- Culture Media MeSH
- Acids MeSH
Microplate readers have been useful assistants of researchers for several decades. This work is focused on the applications of a simple absorbance microplate reader in yeast physiology research, and its advantages and limitations in comparison with alternative methods are discussed. The two main procedures involved are measuring growth curves and monitoring the pH changes of medium using two different pH indicators. We suggest mathematical formulas for converting absorbance data into pH values. With a microplate reader as many as 96 samples can be simultaneously analyzed, while medium consumption is minimized to 100 microL per sample. The results can be observed in 24-48 h (for growth curves) or in 1-3 h (for pH changes) with minimal hands-on time required.
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