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Article

PubMed

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Molecular evidence of Bartonella DNA in ixodid ticks in Czechia

Folia microbiologica. 2007 ; 52 (5) : 503-9.

Folia Microbiol (Praha)
ISSN 0015-5632
Source

Language English Country United States Media print

Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid18298048

PubMed 18298048
DOI 10.1007/bf02932111
Knihovny.cz E-resources

MeSH
Bartonella genetics isolation & purification MeSH
DNA, Bacterial genetics MeSH
DNA, Intergenic genetics MeSH
Ixodidae classification microbiology MeSH
Humans MeSH
Oligonucleotide Probes genetics MeSH
Polymerase Chain Reaction methods MeSH
DNA, Ribosomal genetics MeSH
RNA, Ribosomal, 16S genetics MeSH
RNA, Ribosomal, 23S genetics MeSH
Sensitivity and Specificity MeSH
Blotting, Southern methods MeSH
Animals MeSH
Check Tag
Humans MeSH
Male MeSH
Female MeSH
Animals MeSH
Publication type
Journal Article MeSH
Evaluation Study MeSH
Research Support, Non-U.S. Gov't MeSH
Geographicals
Czech Republic MeSH
Names of Substances
DNA, Bacterial MeSH
DNA, Intergenic MeSH
Oligonucleotide Probes MeSH
DNA, Ribosomal MeSH
RNA, Ribosomal, 16S MeSH
RNA, Ribosomal, 23S MeSH

We report the development of a novel method for detection of Bartonella DNA in ixodid ticks. The assay is based on a specific amplification of a part of 16S rRNA gene and 16S-23S rRNA intergenic spacer region of Bartonella sp. by nested PCR and Southern blot hybridization with specific DNA probe; the method is highly sensitive and specific. The screening of 327 unfed ticks collected in different urban and suburban areas of Czechia in 2003-2005 revealed the presence of Bartonella DNA in four Ixodes ricinus individuals (1.2%), two males, one female and one nymph.

Laboratory of Cell Signaling Cell and Molecular Microbiology Division Institute ofMicrobiology Academy of Sciences of the Czech Republic 142 20 Prague Czechia

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