In vitro ageing of pig oocytes: effects of the histone deacetylase inhibitor trichostatin A
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18405435
DOI
10.1017/s0967199408004668
PII: S0967199408004668
Knihovny.cz E-resources
- MeSH
- Histone Deacetylases metabolism MeSH
- Enzyme Inhibitors pharmacology MeSH
- Histone Deacetylase Inhibitors * MeSH
- Protein Synthesis Inhibitors pharmacology MeSH
- Ionophores pharmacology MeSH
- Cells, Cultured MeSH
- Hydroxamic Acids pharmacology MeSH
- Oocytes enzymology physiology MeSH
- Swine physiology MeSH
- Cellular Senescence MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Histone Deacetylases MeSH
- Enzyme Inhibitors MeSH
- Histone Deacetylase Inhibitors * MeSH
- Protein Synthesis Inhibitors MeSH
- Ionophores MeSH
- Hydroxamic Acids MeSH
- trichostatin A MeSH Browser
After in vitro maturation, the unfertilized pig oocytes underwent the process called ageing. This process involves typical events such as fragmentation, spontaneous parthenogenetic activation or lysis. Inhibition of histone deacetylase, using its specific inhibitor trichostatin A (TSA), significantly delayed the maturation of pig oocytes cultured in vitro. The ageing of oocytes matured under the effect of TSA is the same as the ageing in oocytes matured without TSA. The inhibition of histone deacetylase during oocyte ageing significantly reduced the percentage of fragmented oocytes (from 30% in untreated oocytes to 9% in oocytes aged under the effect of 100 nM of TSA). Oocytes matured in vitro and subsequently aged for 1 day under the effects of TSA retained their developmental capacity. After parthenogenetic activation, a significantly higher portion (27% vs. 15%) of oocytes developed to the blastocyst stage after 24 h ageing under 100 nM TSA when compared with oocytes activated after 24 h ageing in a TSA-free medium. The parthenogenetic development in oocytes aged under TSA treatment is similar to the development of fresh oocytes (29% of blastocyst) artificially activated immediately after in vitro maturation.
References provided by Crossref.org
Involvement of K+ATP and Ca2+ channels in hydrogen sulfide-suppressed ageing of porcine oocytes
Anti-apoptotic properties of carbon monoxide in porcine oocyte during in vitro aging
