Vascular endothelial cells on two-and three-dimensional fibrin assemblies for biomaterial coatings
Language English Country United States Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
18481789
DOI
10.1002/jbm.a.32065
Knihovny.cz E-resources
- MeSH
- Adsorption MeSH
- Actins metabolism MeSH
- Antithrombin III metabolism MeSH
- Coated Materials, Biocompatible * chemistry metabolism MeSH
- Cell Culture Techniques * MeSH
- Endothelial Cells cytology metabolism MeSH
- Fibrin metabolism ultrastructure MeSH
- Fibrinogen metabolism MeSH
- Gels chemistry metabolism MeSH
- Heparin metabolism MeSH
- Integrin alphaV metabolism MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Polyesters chemistry MeSH
- Surface Properties MeSH
- Cattle MeSH
- Materials Testing MeSH
- Tissue Scaffolds chemistry MeSH
- Thrombin metabolism MeSH
- Vinculin metabolism MeSH
- von Willebrand Factor metabolism MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Antithrombin III MeSH
- Coated Materials, Biocompatible * MeSH
- Fibrin MeSH
- Fibrinogen MeSH
- Gels MeSH
- Heparin MeSH
- Integrin alphaV MeSH
- poly(lactide) MeSH Browser
- Polyesters MeSH
- Thrombin MeSH
- Vinculin MeSH
- von Willebrand Factor MeSH
Various techniques for coating synthetic surfaces with fibrin structures were tested for seeding bovine pulmonary artery endothelial cells (EC). Two-dimensional fibrin (Fb) structures (2D Fb) were obtained by successively repeating adsorption of fibrinogen (Fbg), incubating the surface with thrombin (Thr) solution, and inhibiting surface-attached Thr. Three-dimensional fibrin networks immobilized at the surface (3D Fb) were formed by catalytic action of surface-attached thrombin on an ambient Fbg solution. Ultra-thin 3D Fbs were obtained if thrombin inhibitors antithrombin III and heparin were added into an Fbg solution. The formation of surface fibrin structures was observed in situ using surface plasmon resonance. The morphology of the structures was studied by transmission and scanning electron microscopy. A polylactide fibrous scaffold was modified with a surface fibrin film without filling the inner pores with a bulk gel. The growth of EC seeded on a polystyrene surface coated with the Fb films was evaluated by the number and morphology of the adhering ECs and the concentration of beta-actin, vinculin, alpha(v)-intergrin, and von Willebrand factor (vWF). The best initial cell spreading after 1 day was observed on 2D Fb and ultra-thin 3D Fb. The highest concentration of vWF, a marker of EC differentiation, was observed after 3 days on thick 3D Fbs. The highest EC population densities after 7 days were observed on 2D Fb and thick 3D Fb.
References provided by Crossref.org
Cellular Responses Modulated by FGF-2 Adsorbed on Albumin/Heparin Layer-by-Layer Assemblies
