Nuclear organization of PML bodies in leukaemic and multiple myeloma cells
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
18534676
DOI
10.1016/j.leukres.2008.04.021
PII: S0145-2126(08)00225-7
Knihovny.cz E-zdroje
- MeSH
- akutní promyelocytární leukemie patologie MeSH
- buněčná diferenciace účinky léků MeSH
- buněčné jádro patologie MeSH
- buněčný cyklus fyziologie MeSH
- buňky K562 účinky léků patologie účinky záření MeSH
- HL-60 buňky účinky léků patologie účinky záření MeSH
- intranukleární inkluzní tělíska účinky léků patologie účinky záření MeSH
- lidé MeSH
- melfalan farmakologie MeSH
- mnohočetný myelom patologie MeSH
- nádorové buněčné linie MeSH
- průtoková cytometrie MeSH
- tetradekanoylforbolacetát farmakologie MeSH
- tretinoin farmakologie MeSH
- U937 buňky patologie MeSH
- záření gama MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- melfalan MeSH
- tetradekanoylforbolacetát MeSH
- tretinoin MeSH
The nuclear arrangement of promyelocytic leukaemia nuclear bodies (PML NBs) was studied in vitro after the cell treatment by clinically used agents such as all-trans retinoic acid (RA) in human leukaemia and cytostatics or gamma radiation in multiple myeloma cells. In addition, the influence of phorbol ester (PMA) on PML NBs formation was analyzed. A reduced number of PML bodies, which led to relocation of PML NBs closer to the nuclear interior, mostly accompanied RA- and PMA-induced differentiation. Centrally located PML NBs were associated with transcriptional protein RNAP II and SC35 regions, which support importance of PML NBs in RNA processing that mostly proceeds within the nuclear interior. Conversely, the quantity of PML NBs was increased after cytostatic treatment, which caused re-distribution of PML NBs closer to the nuclear envelope. Here we showed correlations between the number of PML NBs and average Centre-to-PML distances. Moreover, a number of cells in S phase, especially during differentiation, influenced number of PML NBs. Studying the proteins involved in PML compartment, such as c-MYC, cell-type specific association of c-MYC and the PML NBs was observed in selected leukaemic cells undergoing differentiation, which was accompanied by c-MYC down-regulation.
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