Building of RNA and DNA double helices into electron density
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Validation Study
PubMed
18560149
DOI
10.1107/s0907444908007075
PII: S0907444908007075
Knihovny.cz E-resources
- MeSH
- DNA chemistry MeSH
- Nucleic Acid Conformation * MeSH
- Crystallography, X-Ray MeSH
- Models, Molecular MeSH
- Base Pairing MeSH
- Computer Simulation MeSH
- RNA chemistry MeSH
- Software MeSH
- Static Electricity MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Comparative Study MeSH
- Validation Study MeSH
- Names of Substances
- DNA MeSH
- RNA MeSH
A method has been developed that automatically fits double-helical regions into the electron density of nucleic acid structures. Rigid fragments consisting of two Watson-Crick base pairs and three pairs of phosphate groups in the A-type or B-type conformation are positioned into the electron density by phased rotation and translation functions. The position and orientation of the localized double-helical fragments are determined by phased refinement. The method has been tested by building double-helical regions of nine RNA structures of variable crystallographic resolution and polynucleotide length and is available for free use.
References provided by Crossref.org
