Building of RNA and DNA double helices into electron density

. 2008 Jun ; 64 (Pt 6) : 620-6. [epub] 20080514

Jazyk angličtina Země Spojené státy americké Médium print-electronic

Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S., validační studie

Perzistentní odkaz   https://www.medvik.cz/link/pmid18560149

A method has been developed that automatically fits double-helical regions into the electron density of nucleic acid structures. Rigid fragments consisting of two Watson-Crick base pairs and three pairs of phosphate groups in the A-type or B-type conformation are positioned into the electron density by phased rotation and translation functions. The position and orientation of the localized double-helical fragments are determined by phased refinement. The method has been tested by building double-helical regions of nine RNA structures of variable crystallographic resolution and polynucleotide length and is available for free use.

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