The intestinal microflora of childhood patients with indicated celiac disease
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bacteria classification genetics isolation & purification MeSH
- Biopsy MeSH
- Celiac Disease microbiology MeSH
- Child MeSH
- DNA, Bacterial analysis isolation & purification MeSH
- Duodenum microbiology MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Feces chemistry microbiology MeSH
- Infant MeSH
- Hydrogen-Ion Concentration MeSH
- Fatty Acids, Volatile analysis MeSH
- Humans MeSH
- Adolescent MeSH
- Polymerase Chain Reaction methods MeSH
- Child, Preschool MeSH
- Intestines microbiology MeSH
- Check Tag
- Child MeSH
- Infant MeSH
- Humans MeSH
- Adolescent MeSH
- Child, Preschool MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Fatty Acids, Volatile MeSH
The fecal short-chain fatty acids concentration was higher (154 +/- 46.9 mmol/L) in childhood patients than in healthy children (96.6 +/- 19.2 mmol/L). On the other hand, pH values were nonsignificantly lower in patients stool (6.78 +/- 0.75 vs. children 7.42 +/- 0.74). Using denaturing gradient gel electrophoresis specific for total bacteria, lactobacilli and bifidobacteria the microbial population was characterized in fecal samples and in duodenal biopsies. Bacteria adhering to duodenal biopsies were not dominating in stool samples. More than 50 % of detected bacterial species belonged to as yet uncultured strains.
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