Oxidative DNA damage in bone marrow cells of patients with low-risk myelodysplastic syndrome
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18687469
DOI
10.1016/j.leukres.2008.07.005
PII: S0145-2126(08)00332-9
Knihovny.cz E-resources
- MeSH
- Bone Marrow Cells pathology MeSH
- Comet Assay MeSH
- Middle Aged MeSH
- Humans MeSH
- Myelodysplastic Syndromes pathology MeSH
- Oxidative Stress MeSH
- DNA Damage * MeSH
- Anemia, Refractory, with Excess of Blasts MeSH
- Anemia, Refractory MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Case-Control Studies MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Bone marrow aspirates of 19 patients with low-risk myelodysplastic syndromes (MDS) and 14 control subjects were collected in order to assess the level of oxidative DNA damage. Glycophorin A positive and negative cells separated by miniMACS magnetic cell sorting were subjected to single cell gel electrophoresis (comet assay) combined with enzymes of base excision repair (endonuclease III and formamido-pyrimidine-glycosylase) that specifically recognize oxidized nucleotides. Compared to controls, MDS patients exhibited a significant increase of oxidative damage to DNA which could contribute to genomic instability and disease progression.
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